1en4: Difference between revisions

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New page: left|200px<br /><applet load="1en4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1en4, resolution 2.0Å" /> '''CRYSTAL STRUCTURE ANA...
 
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[[Image:1en4.jpg|left|200px]]<br /><applet load="1en4" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1en4, resolution 2.0&Aring;" />
'''CRYSTAL STRUCTURE ANALYSIS OF THE E. COLI MANGANESE SUPEROXIDE DISMUTASE Q146H MUTANT'''<br />


==Overview==
==CRYSTAL STRUCTURE ANALYSIS OF THE E. COLI MANGANESE SUPEROXIDE DISMUTASE Q146H MUTANT==
Tyrosine 34 and glutamine 146 are highly conserved outer sphere residues, in the mononuclear manganese active site of Escherichia coli manganese, superoxide dismutase. Biochemical and spectroscopic characterization of, site-directed mutants has allowed functional characterization of these, residues in the wild-type (wt) enzyme. X-ray crystallographic analysis of, three mutants (Y34F, Q146L, and Q146H) reveal subtle changes in the, protein structures. The Y34A mutant, as well as the previously reported, Y34F mutant, retained essentially the full superoxide dismutase activity, of the wild-type enzyme, and the X-ray crystal structure of Y34F manganese, superoxide dismutase shows that mutation of this strictly conserved, residue has only minor effects on the positions of active site residues, and the organized water in the substrate access funnel. Mutation of the, outer sphere solvent pocket residue Q146 has more dramatic effects. The, Q146E mutant is isolated as an apoprotein lacking dismutase activity., Q146L and Q146H mutants retain only 5-10% of the dismutase activity of the, wild-type enzyme. The absorption and circular dichroism spectra of the, Q146H mutant resemble corresponding data for the superoxide dismutase from, a hyperthermophilic archaeon, Pyrobaculum aerophilum, which is active in, both Mn and Fe forms. Interestingly, the iron-substituted Q146H protein, also exhibits low dismutase activity, which increases at lower pH., Mutation of glutamine 146 disrupts the hydrogen-bonding network in the, active site and has a greater effect on protein structure than does the, Y34F mutant, with rearrangement of the tyrosine 34 and tryptophan 128 side, chains.
<StructureSection load='1en4' size='340' side='right'caption='[[1en4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1en4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EN4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1en4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1en4 OCA], [https://pdbe.org/1en4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1en4 RCSB], [https://www.ebi.ac.uk/pdbsum/1en4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1en4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SODM_ECOLI SODM_ECOLI] Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/en/1en4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1en4 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1EN4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EN4 OCA].
*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Outer sphere mutations perturb metal reactivity in manganese superoxide dismutase., Edwards RA, Whittaker MM, Whittaker JW, Baker EN, Jameson GB, Biochemistry. 2001 Jan 9;40(1):15-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11141052 11141052]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Superoxide dismutase]]
[[Category: Baker EN]]
[[Category: Baker, E.N.]]
[[Category: Edwards RA]]
[[Category: Edwards, R.A.]]
[[Category: Jameson GB]]
[[Category: Jameson, G.B.]]
[[Category: Whittaker JW]]
[[Category: Whittaker, J.W.]]
[[Category: Whittaker MM]]
[[Category: Whittaker, M.M.]]
[[Category: MN]]
[[Category: manganese superoxide dismutase]]
[[Category: mutant]]
[[Category: proton shuttle]]
[[Category: q146h]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:08:18 2007''

Latest revision as of 10:04, 7 February 2024

CRYSTAL STRUCTURE ANALYSIS OF THE E. COLI MANGANESE SUPEROXIDE DISMUTASE Q146H MUTANTCRYSTAL STRUCTURE ANALYSIS OF THE E. COLI MANGANESE SUPEROXIDE DISMUTASE Q146H MUTANT

Structural highlights

1en4 is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SODM_ECOLI Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1en4, resolution 2.00Å

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