1emy: Difference between revisions

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-[[Image:1emy.gif|left|200px]]
- {{Structure
+==CRYSTAL STRUCTURE OF ASIAN ELEPHANT (ELEPHAS MAXIMUS) CYANO-MET MYOGLOBIN AT 1.78 ANGSTROMS RESOLUTION. PHE 29 (B10) ACCOUNTS FOR ITS UNUSUAL LIGAND BINDING PROPERTIES==
-|PDB= 1emy |SIZE=350|CAPTION= <scene name='initialview01'>1emy</scene>, resolution 1.78&Aring;
+<StructureSection load='1emy' size='340' side='right'caption='[[1emy]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
+<table><tr><td colspan='2'>[[1emy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Elephas_maximus Elephas maximus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EMY FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1emy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1emy OCA], [https://pdbe.org/1emy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1emy RCSB], [https://www.ebi.ac.uk/pdbsum/1emy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1emy ProSAT]</span></td></tr>
-|RELATEDENTRY=
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1emy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1emy OCA], [http://www.ebi.ac.uk/pdbsum/1emy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1emy RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/MYG_ELEMA MYG_ELEMA] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/1emy_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1emy ConSurf].
+<div style="clear:both"></div>
-'''CRYSTAL STRUCTURE OF ASIAN ELEPHANT (ELEPHAS MAXIMUS) CYANO-MET MYOGLOBIN AT 1.78 ANGSTROMS RESOLUTION. PHE 29 (B10) ACCOUNTS FOR ITS UNUSUAL LIGAND BINDING PROPERTIES'''
+==See Also==
+*[[Myoglobin 3D structures|Myoglobin 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-The crystal structure of Asian elephant cyano-metmyoglobin which has a glutamine instead of the usual distal site histidine has been determined to high resolution. In addition to this replacement, the substitution of a conserved leucine residue in position 29(B10) at the distal side by a phenylalanine was unambiguously identified based on the available electron density. The suspicion, that there were errors in the original sequence which has caused some confusion, is thus confirmed. Comparison with other myoglobin structures in various ligated forms reveals an essentially unchanged tertiary structure in elephant myoglobin despite the two amino acid substitutions in the heme pocket. Our current structural model shows that the N epsilon 2 atom of Gln64(E7) has moved with respect to the corresponding nitrogen position of His64(E7) in the CO complex of sperm whale myoglobin. The newly assigned residue Phe29(B10) penetrates into the distal side of the heme pocket approaching the ligand within van der Waals distance and causing a much more crowded heme pocket compared to other myoglobins. Kinetic properties of Asian elephant myoglobin, wild type, and recombinant sperm whale myoglobins are discussed in relation to the structural consequences of the two amino acid substitutions H64Q and L29F.
-==About this Structure==
-EMY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Elephas_maximus Elephas maximus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMY OCA].
-==Reference==
-Crystal structure of Asian elephant (Elephas maximus) cyano-metmyoglobin at 1.78-A resolution. Phe29(B10) accounts for its unusual ligand binding properties., Bisig DA, Di Iorio EE, Diederichs K, Winterhalter KH, Piontek K, J Biol Chem. 1995 Sep 1;270(35):20754-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7657658 7657658]
 [[Category: Elephas maximus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Bisig, D A.]]
+[[Category: Bisig DA]]
-[[Category: Piontek, K.]]
+[[Category: Piontek K]]
-[[Category: globin fold]]
-[[Category: heme protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:05:44 2008''