1el6: Difference between revisions

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OCA

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-[[Image:1el6.gif|left|200px]]<br /><applet load="1el6" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1el6, resolution 2.0&Aring;" />
-'''STRUCTURE OF BACTERIOPHAGE T4 GENE PRODUCT 11, THE INTERFACE BETWEEN THE BASEPLATE AND SHORT TAIL FIBERS'''<br />
-==Overview==
+==STRUCTURE OF BACTERIOPHAGE T4 GENE PRODUCT 11, THE INTERFACE BETWEEN THE BASEPLATE AND SHORT TAIL FIBERS==
-Bacteriophage T4, like all other viruses, is required to be stable while, being transmitted from host to host, but also is poised to eject, efficiently and rapidly its double-stranded DNA genome to initiate, infection. The latter is coordinated by the recognition of receptors on, Escherichia coli cells by the long tail fibers and subsequent irreversible, attachment by the short tail fibers. These fibers are attached to the, baseplate, a multi-subunit assembly at the distal end of the tail., Recognition and attachment induce a conformational transition of the, baseplate from a hexagonal to a star-shaped structure.The crystal, structure of gene product 11 (gp11), a protein that connects the short, tail fibers to the baseplate, has been determined to 2.0 A resolution, using multiple wavelength anomalous dispersion with Se. This structure is, compared to the trimeric structure of gp9, which connects the baseplate, with the long tail fibers. The structure of gp11 is a trimer with each, monomer consisting of 218 residues folded into three domains. The, N-terminal domains form a central, trimeric, parallel coiled coil, surrounded by the middle "finger" domains. The fingers emanate from the, carboxy-terminal beta-annulus domain, which, by comparison with the T4, whisker "fibritin" protein, is probably responsible for trimerization. The, events leading from recognition of the host to the ejection of viral DNA, must be communicated along the assembled trimeric (gp9)(3) attached to the, long tail fibers via the trimeric baseplate protein (gp10)(3) to the, trimeric (gp11)(3) and the trimeric short tail fibers.
+<StructureSection load='1el6' size='340' side='right'caption='[[1el6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[1el6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EL6 FirstGlance]. <br>
-EL6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EL6 OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1el6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1el6 OCA], [https://pdbe.org/1el6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1el6 RCSB], [https://www.ebi.ac.uk/pdbsum/1el6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1el6 ProSAT]</span></td></tr>
-==Reference==
+</table>
-Structure of bacteriophage T4 gene product 11, the interface between the baseplate and short tail fibers., Leiman PG, Kostyuchenko VA, Shneider MM, Kurochkina LP, Mesyanzhinov VV, Rossmann MG, J Mol Biol. 2000 Aug 25;301(4):975-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10966799 10966799]
+== Function ==
-[[Category: Bacteriophage t4]]
+[https://www.uniprot.org/uniprot/BP11_BPT4 BP11_BPT4] Baseplate protein that is part of the baseplate wedge and that connects the short tail fibers to the baseplate (PubMed:15315755). Involved in the tail assembly.<ref>PMID:15315755</ref> <ref>PMID:21129200</ref>
-[[Category: Single protein]]
+== Evolutionary Conservation ==
-[[Category: Kostyuchenko, V.A.]]
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Kurochkina, L.P.]]
+Check<jmol>
-[[Category: Leiman, P.G.]]
+  <jmolCheckbox>
-[[Category: Mesyanzhinov, V.V.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/1el6_consurf.spt"</scriptWhenChecked>
-[[Category: Rossmann, M.G.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Schneider, M.M.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: a fiber attachment protein]]
+  </jmolCheckbox>
-[[Category: beta annulus]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1el6 ConSurf].
-[[Category: coiled coil]]
+<div style="clear:both"></div>
+== References ==
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:05:55 2007''
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia virus T4]]
+[[Category: Large Structures]]
+[[Category: Kostyuchenko VA]]
+[[Category: Kurochkina LP]]
+[[Category: Leiman PG]]
+[[Category: Mesyanzhinov VV]]
+[[Category: Rossmann MG]]
+[[Category: Schneider MM]]