1ej4: Difference between revisions

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New page: left|200px<br /><applet load="1ej4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ej4, resolution 2.25Å" /> '''COCRYSTAL STRUCTURE ...
 
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[[Image:1ej4.jpg|left|200px]]<br /><applet load="1ej4" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ej4, resolution 2.25&Aring;" />
'''COCRYSTAL STRUCTURE OF EIF4E/4E-BP1 PEPTIDE'''<br />


==Overview==
==COCRYSTAL STRUCTURE OF EIF4E/4E-BP1 PEPTIDE==
eIF4G uses a conserved Tyr-X-X-X-X-Leu-phi segment (where X is variable, and phi is hydrophobic) to recognize eIF4E during cap-dependent, translation initiation in eukaryotes. High-resolution X-ray, crystallography and complementary biophysical methods have revealed that, this eIF4E recognition motif undergoes a disorder-to-order transition, adopting an L-shaped, extended chain/alpha-helical conformation when it, interacts with a phylogenetically invariant portion of the convex surface, of eIF4E. Inhibitors of translation initiation known as eIF4E-binding, proteins (4E-BPs) contain similar eIF4E recognition motifs. These, molecules are molecular mimics of eIF4G, which act by occupying the same, binding site on the convex dorsum of eIF4E and blocking assembly of the, translation machinery. The implications of our results for translation, initiation are discussed in detail, and a molecular mechanism for relief, of translation inhibition following phosphorylation of the 4E-BPs is, proposed.
<StructureSection load='1ej4' size='340' side='right'caption='[[1ej4]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ej4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EJ4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M7G:7N-METHYL-8-HYDROGUANOSINE-5-DIPHOSPHATE'>M7G</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ej4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ej4 OCA], [https://pdbe.org/1ej4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ej4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ej4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ej4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IF4E_MOUSE IF4E_MOUSE] Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity). Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.<ref>PMID:18805096</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/1ej4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ej4 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1EJ4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with M7G as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EJ4 OCA].
*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
 
== References ==
==Reference==
<references/>
Cap-dependent translation initiation in eukaryotes is regulated by a molecular mimic of eIF4G., Marcotrigiano J, Gingras AC, Sonenberg N, Burley SK, Mol Cell. 1999 Jun;3(6):707-16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10394359 10394359]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Burley SK]]
[[Category: Burley, S.K.]]
[[Category: Gingras A-C]]
[[Category: Gingras, A.C.]]
[[Category: Marcotrigiano J]]
[[Category: Marcotrigiano, J.]]
[[Category: Sonenberg N]]
[[Category: Sonenberg, N.]]
[[Category: M7G]]
[[Category: eif4e/4e-bp/7-methyl-gdp]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 14:02:40 2007''

Latest revision as of 10:03, 7 February 2024

COCRYSTAL STRUCTURE OF EIF4E/4E-BP1 PEPTIDECOCRYSTAL STRUCTURE OF EIF4E/4E-BP1 PEPTIDE

Structural highlights

1ej4 is a 2 chain structure with sequence from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.25Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IF4E_MOUSE Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity). Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

References

  1. ↑ Napoli I, Mercaldo V, Boyl PP, Eleuteri B, Zalfa F, De Rubeis S, Di Marino D, Mohr E, Massimi M, Falconi M, Witke W, Costa-Mattioli M, Sonenberg N, Achsel T, Bagni C. The fragile X syndrome protein represses activity-dependent translation through CYFIP1, a new 4E-BP. Cell. 2008 Sep 19;134(6):1042-54. doi: 10.1016/j.cell.2008.07.031. PMID:18805096 doi:http://dx.doi.org/10.1016/j.cell.2008.07.031

1ej4, resolution 2.25Å

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