1ei7: Difference between revisions

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[[Image:1ei7.jpg|left|200px]]


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==TMV COAT PROTEIN REFINED FROM THE 4-LAYER AGGREGATE==
The line below this paragraph, containing "STRUCTURE_1ei7", creates the "Structure Box" on the page.
<StructureSection load='1ei7' size='340' side='right'caption='[[1ei7]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1ei7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tobacco_mosaic_virus Tobacco mosaic virus]. The January 2009 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Tobacco Mosaic Virus''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2009_1 10.2210/rcsb_pdb/mom_2009_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EI7 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ei7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ei7 OCA], [https://pdbe.org/1ei7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ei7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ei7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ei7 ProSAT]</span></td></tr>
{{STRUCTURE_1ei7|  PDB=1ei7 |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAPSD_TMV CAPSD_TMV] Capsid protein self-assembles to form rod-shaped virions about 18 nm in diameter with a central canal enclosing the viral genomic RNA.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1ei7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ei7 ConSurf].
<div style="clear:both"></div>


'''TMV COAT PROTEIN REFINED FROM THE 4-LAYER AGGREGATE'''
==See Also==
 
*[[Tobacco Mosaic Virus|Tobacco Mosaic Virus]]
 
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
==Overview==
__TOC__
Previous x-ray studies (2.8-A resolution) on crystals of tobacco mosaic virus coat protein grown from solutions containing high salt have characterized the structure of the protein aggregate as a dimer of a bilayered cylindrical disk formed by 34 chemically identical subunits. We have determined the crystal structure of the disk aggregate at 2.4-A resolution using x-ray diffraction from crystals maintained at cryogenic temperatures. Two regions of interest have been extensively refined. First, residues of the low-radius loop region, which were not modeled previously, have been traced completely in our electron density maps. Similar to the structure observed in the virus, the right radial helix in each protomer ends around residue 87, after which the protein chain forms an extended chain that extends to the left radial helix. The left radial helix appears as a long alpha-helix with high temperature factors for the main-chain atoms in the inner portion. The side-chain atoms in this region (residues 90-110) are not visible in the electron density maps and are assumed to be disordered. Second, interactions between subunits in the symmetry-related central A pair have been determined. No direct protein-protein interactions are observed in the major overlap region between these subunits; all interactions are mediated by two layers of ordered solvent molecules. The current structure emphasizes the importance of water in biological macromolecular assemblies.
</StructureSection>
 
[[Category: Large Structures]]
==About this Structure==
[[Category: RCSB PDB Molecule of the Month]]
1EI7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tobacco_mosaic_virus Tobacco mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EI7 OCA].
[[Category: Tobacco Mosaic Virus]]
 
==Reference==
Refined atomic model of the four-layer aggregate of the tobacco mosaic virus coat protein at 2.4-A resolution., Bhyravbhatla B, Watowich SJ, Caspar DL, Biophys J. 1998 Jan;74(1):604-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9449361 9449361]
[[Category: Single protein]]
[[Category: Tobacco mosaic virus]]
[[Category: Tobacco mosaic virus]]
[[Category: Bhyravbhatla, B.]]
[[Category: Bhyravbhatla B]]
[[Category: Caspar, D L.]]
[[Category: Caspar DL]]
[[Category: Watowich, S J.]]
[[Category: Watowich SJ]]
[[Category: Disordered loop]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:07:56 2008''

Latest revision as of 10:03, 7 February 2024

TMV COAT PROTEIN REFINED FROM THE 4-LAYER AGGREGATETMV COAT PROTEIN REFINED FROM THE 4-LAYER AGGREGATE

Structural highlights

1ei7 is a 2 chain structure with sequence from Tobacco mosaic virus. The January 2009 RCSB PDB Molecule of the Month feature on Tobacco Mosaic Virus by David Goodsell is 10.2210/rcsb_pdb/mom_2009_1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.45Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CAPSD_TMV Capsid protein self-assembles to form rod-shaped virions about 18 nm in diameter with a central canal enclosing the viral genomic RNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1ei7, resolution 2.45Å

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