1eg7: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(11 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1eg7.jpg|left|200px]]


<!--
==THE CRYSTAL STRUCTURE OF FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA==
The line below this paragraph, containing "STRUCTURE_1eg7", creates the "Structure Box" on the page.
<StructureSection load='1eg7' size='340' side='right'caption='[[1eg7]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1eg7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EG7 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
{{STRUCTURE_1eg7|  PDB=1eg7  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eg7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eg7 OCA], [https://pdbe.org/1eg7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eg7 RCSB], [https://www.ebi.ac.uk/pdbsum/1eg7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eg7 ProSAT]</span></td></tr>
 
</table>
'''THE CRYSTAL STRUCTURE OF FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA'''
== Function ==
 
[https://www.uniprot.org/uniprot/FTHS_MOOTH FTHS_MOOTH]
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
The structure was solved at 2.5 A resolution using multiwavelength anomalous dispersion (MAD) scattering by Se-Met residues. The subunit of N(10)-formyltetrahydrofolate synthetase is composed of three domains organized around three mixed beta-sheets. There are two cavities between adjacent domains. One of them was identified as the nucleotide binding site by homology modeling. The large domain contains a seven-stranded beta-sheet surrounded by helices on both sides. The second domain contains a five-stranded beta-sheet with two alpha-helices packed on one side while the other two are a wall of the active site cavity. The third domain contains a four-stranded beta-sheet forming a half-barrel. The concave side is covered by two helices while the convex side is another wall of the large cavity. Arg 97 is likely involved in formyl phosphate binding. The tetrameric molecule is relatively flat with the shape of the letter X, and the active sites are located at the end of the subunits far from the subunit interface.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1eg7_consurf.spt"</scriptWhenChecked>
1EG7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Moorella_thermoacetica Moorella thermoacetica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG7 OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica., Radfar R, Shin R, Sheldrick GM, Minor W, Lovell CR, Odom JD, Dunlap RB, Lebioda L, Biochemistry. 2000 Apr 11;39(14):3920-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10747779 10747779]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eg7 ConSurf].
[[Category: Formate--tetrahydrofolate ligase]]
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Moorella thermoacetica]]
[[Category: Moorella thermoacetica]]
[[Category: Single protein]]
[[Category: Dunlap RB]]
[[Category: Dunlap, R B.]]
[[Category: Lebioda L]]
[[Category: Lebioda, L.]]
[[Category: Lovell CR]]
[[Category: Lovell, C R.]]
[[Category: Minor W]]
[[Category: Minor, W.]]
[[Category: Odom JD]]
[[Category: Odom, J D.]]
[[Category: Radfar R]]
[[Category: Radfar, R.]]
[[Category: Sheldrick GM]]
[[Category: Sheldrick, G M.]]
[[Category: Shin R]]
[[Category: Shin, R.]]
[[Category: Atp binding]]
[[Category: Folate binding]]
[[Category: Formate binding]]
[[Category: Monovalent cation binding]]
[[Category: Synthetase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 15:03:47 2008''

Latest revision as of 10:02, 7 February 2024

THE CRYSTAL STRUCTURE OF FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICATHE CRYSTAL STRUCTURE OF FORMYLTETRAHYDROFOLATE SYNTHETASE FROM MOORELLA THERMOACETICA

Structural highlights

1eg7 is a 2 chain structure with sequence from Moorella thermoacetica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FTHS_MOOTH

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1eg7, resolution 2.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1eg7&oldid=4042539"