1eg2: Difference between revisions

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[[Image:1eg2.png|left|200px]]


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==CRYSTAL STRUCTURE OF RHODOBACTER SPHEROIDES (N6 ADENOSINE) METHYLTRANSFERASE (M.RSRI)==
The line below this paragraph, containing "STRUCTURE_1eg2", creates the "Structure Box" on the page.
<StructureSection load='1eg2' size='340' side='right'caption='[[1eg2]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1eg2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EG2 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTA:5-DEOXY-5-METHYLTHIOADENOSINE'>MTA</scene></td></tr>
{{STRUCTURE_1eg2|  PDB=1eg2  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eg2 OCA], [https://pdbe.org/1eg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eg2 RCSB], [https://www.ebi.ac.uk/pdbsum/1eg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eg2 ProSAT], [https://www.topsan.org/Proteins/MCSG/1eg2 TOPSAN]</span></td></tr>
 
</table>
===CRYSTAL STRUCTURE OF RHODOBACTER SPHEROIDES (N6 ADENOSINE) METHYLTRANSFERASE (M.RSRI)===
== Function ==
 
[https://www.uniprot.org/uniprot/MTR1_CERSP MTR1_CERSP] A beta subtype methylase, recognizes the double-stranded sequence 5'-GAATTC-3', methylates A-3 on both strands, and protects the DNA from cleavage by the RsrI endonuclease.<ref>PMID:2690017</ref> <ref>PMID:12654995</ref>
 
== Evolutionary Conservation ==
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Check<jmol>
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    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eg/1eg2_consurf.spt"</scriptWhenChecked>
{{ABSTRACT_PUBMED_11024175}}
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    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1EG2 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EG2 OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eg2 ConSurf].
 
<div style="clear:both"></div>
==Reference==
== References ==
<ref group="xtra">PMID:11024175</ref><references group="xtra"/>
<references/>
[[Category: Rhodobacter sphaeroides]]
__TOC__
[[Category: Churchill, M E.A.]]
</StructureSection>
[[Category: Gumport, R I.]]
[[Category: Cereibacter sphaeroides]]
[[Category: Joachimiak, A.]]
[[Category: Large Structures]]
[[Category: MCSG, Midwest Center for Structural Genomics.]]
[[Category: Churchill MEA]]
[[Category: Scavetta, R D.]]
[[Category: Gumport RI]]
[[Category: Szegedi, S.]]
[[Category: Joachimiak A]]
[[Category: Thomas, C B.]]
[[Category: Scavetta RD]]
[[Category: Walsh, M A.]]
[[Category: Szegedi S]]
[[Category: Dna binding]]
[[Category: Thomas CB]]
[[Category: Dna methylation]]
[[Category: Walsh MA]]
[[Category: Dna modification]]
[[Category: Exocyclic amino dna methyltransferase rsri]]
[[Category: Mcsg]]
[[Category: Midwest center for structural genomic]]
[[Category: Protein structure initiative]]
[[Category: Psi]]
[[Category: Rossmann fold]]
[[Category: Structural genomic]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 14:16:14 2009''

Latest revision as of 10:02, 7 February 2024

CRYSTAL STRUCTURE OF RHODOBACTER SPHEROIDES (N6 ADENOSINE) METHYLTRANSFERASE (M.RSRI)CRYSTAL STRUCTURE OF RHODOBACTER SPHEROIDES (N6 ADENOSINE) METHYLTRANSFERASE (M.RSRI)

Structural highlights

1eg2 is a 1 chain structure with sequence from Cereibacter sphaeroides. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.75Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

MTR1_CERSP A beta subtype methylase, recognizes the double-stranded sequence 5'-GAATTC-3', methylates A-3 on both strands, and protects the DNA from cleavage by the RsrI endonuclease.[1] [2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Kaszubska W, Aiken C, O'Connor CD, Gumport RI. Purification, cloning and sequence analysis of RsrI DNA methyltransferase: lack of homology between two enzymes, RsrI and EcoRI, that methylate the same nucleotide in identical recognition sequences. Nucleic Acids Res. 1989 Dec 25;17(24):10403-25. PMID:2690017 doi:10.1093/nar/17.24.10403
  2. ↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SKh, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Kruger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY. A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res. 2003 Apr 1;31(7):1805-12. PMID:12654995

1eg2, resolution 1.75Å

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