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New page: left|200px<br /><applet load="1efd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1efd, resolution 1.9Å" /> '''PERIPLASMIC FERRIC SI...
 
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[[Image:1efd.gif|left|200px]]<br /><applet load="1efd" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1efd, resolution 1.9&Aring;" />
'''PERIPLASMIC FERRIC SIDEROPHORE BINDING PROTEIN FHUD COMPLEXED WITH GALLICHROME'''<br />


==Overview==
==PERIPLASMIC FERRIC SIDEROPHORE BINDING PROTEIN FHUD COMPLEXED WITH GALLICHROME==
Siderophore binding proteins play a key role in the uptake of iron in many, gram-positive and gram-negative bacteria. FhuD is a soluble periplasmic, binding protein that transports ferrichrome and other hydroxamate, siderophores. The crystal structure of FhuD from Escherichia coli in, complex with the ferrichrome homolog gallichrome has been determined at, 1.9 inverted question mark resolution, the first structure of a, periplasmic binding protein involved in the uptake of siderophores., Gallichrome is held in a shallow pocket lined with aromatic groups; Arg, and Tyr side chains interact directly with the hydroxamate moieties of the, siderophore. FhuD possesses a novel fold, suggesting that its mechanisms, of ligand binding and release are different from other structurally, characterized periplasmic ligand binding proteins.
<StructureSection load='1efd' size='340' side='right'caption='[[1efd]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1efd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EFD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCR:GALLICHROME'>GCR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1efd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1efd OCA], [https://pdbe.org/1efd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1efd RCSB], [https://www.ebi.ac.uk/pdbsum/1efd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1efd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FHUD_ECOLI FHUD_ECOLI] Part of the ABC transporter complex FhuCDB involved in iron(3+)-hydroxamate import. Binds the iron(3+)-hydroxamate complex and transfers it to the membrane-bound permease. Required for the transport of all iron(3+)-hydroxamate siderophores such as ferrichrome, gallichrome, desferrioxamine, coprogen, aerobactin, shizokinen, rhodotorulic acid and the antibiotic albomycin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ef/1efd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1efd ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1EFD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with GCR as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EFD OCA].
*[[Ferric hydroxamate uptake receptor|Ferric hydroxamate uptake receptor]]
 
__TOC__
==Reference==
</StructureSection>
The structure of the ferric siderophore binding protein FhuD complexed with gallichrome., Clarke TE, Ku SY, Dougan DR, Vogel HJ, Tari LW, Nat Struct Biol. 2000 Apr;7(4):287-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10742172 10742172]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Clarke, T.E.]]
[[Category: Clarke TE]]
[[Category: Dougan, D.R.]]
[[Category: Dougan DR]]
[[Category: Ku, S.Y.]]
[[Category: Ku S-Y]]
[[Category: Tari, L.W.]]
[[Category: Tari LW]]
[[Category: Vogel, H.J.]]
[[Category: Vogel HJ]]
[[Category: GCR]]
[[Category: escherichia coli]]
[[Category: ferric siderophore binding protein]]
[[Category: fhud complex with gallichrome]]
[[Category: periplasmic binding protein-siderophore complex]]
 
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