1ee4: Difference between revisions

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-[[Image:1ee4.gif|left|200px]]<br />
-<applet load="1ee4" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1ee4, resolution 2.1&Aring;" />
-'''CRYSTAL STRUCTURE OF YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A C-MYC NLS PEPTIDE'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF YEAST KARYOPHERIN (IMPORTIN) ALPHA IN A COMPLEX WITH A C-MYC NLS PEPTIDE==
-BACKGROUND: Karyopherin alpha (importin alpha) is an adaptor molecule that, recognizes proteins containing nuclear localization signals (NLSs). The, prototypical NLS that is able to bind to karyopherin alpha is that of the, SV40 T antigen, and consists of a short positively charged sequence motif., Distinct classes of NLSs (monopartite and bipartite) have been identified, that are only partly conserved with respect to one another but are, nevertheless recognized by the same receptor. RESULTS: We report the, crystal structures of two peptide complexes of yeast karyopherin alpha, (Kapalpha): one with a human c-myc NLS peptide, determined at 2.1 A, resolution, and one with a Xenopus nucleoplasmin NLS peptide, determined, at 2.4 A resolution. Analysis of these structures reveals the determinants, of specificity for the binding of a relatively hydrophobic monopartite NLS, and of a bipartite NLS peptide. The peptides bind Kapalpha in its extended, surface groove, which presents a modular array of tandem binding pockets, for amino acid residues. CONCLUSIONS: Monopartite and bipartite NLSs bind, to a different number of amino acid binding pockets and make different, interactions within them. The relatively hydrophobic monopartite c-myc NLS, binds extensively at a few binding pockets in a similar manner to that of, the SV40 T antigen NLS. In contrast, the bipartite nucleoplasmin NLS, engages the whole array of pockets with individually more limited but, overall more abundant interactions, which include the NLS two basic, clusters and the backbone of its non-conserved linker region. Versatility, in the specific recognition of NLSs relies on the modular.
+<StructureSection load='1ee4' size='340' side='right'caption='[[1ee4]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1ee4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EE4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EE4 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ee4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ee4 OCA], [https://pdbe.org/1ee4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ee4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ee4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ee4 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/IMA1_YEAST IMA1_YEAST] Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs (By similarity).<ref>PMID:7565597</ref> <ref>PMID:10913188</ref> <ref>PMID:21075847</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ee/1ee4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ee4 ConSurf].
+<div style="clear:both"></div>
-==Disease==
+==See Also==
-Known disease associated with this structure: Burkitt lymphoma OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=190080 190080]]
+*[[Importin 3D structures|Importin 3D structures]]
+== References ==
-==About this Structure==
+<references/>
-EE4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EE4 OCA].
+__TOC__
+</StructureSection>
-==Reference==
-Crystallographic analysis of the specific yet versatile recognition of distinct nuclear localization signals by karyopherin alpha., Conti E, Kuriyan J, Structure. 2000 Mar 15;8(3):329-38. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10745017 10745017]
 [[Category: Homo sapiens]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
 [[Category: Saccharomyces cerevisiae]]
-[[Category: Conti, E.]]
+[[Category: Conti E]]
-[[Category: arm repeat]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:42:18 2007''