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[[Image:1ecx.gif|left|200px]]<br /><applet load="1ecx" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1ecx, resolution 2.70&Aring;" />
'''NIFS-LIKE PROTEIN'''<br />


==Overview==
==NIFS-LIKE PROTEIN==
NifS-like proteins are ubiquitous, homodimeric, proteins which belong to the alpha-family of pyridoxal-5'-phoshate dependent enzymes. They are proposed to donate elementary sulphur, generated from cysteine, via a cysteinepersulphide intermediate during iron sulphur cluster biosynthesis, an important albeit not well understood process. Here, we report on the crystal structure of a NifS-like protein from the hyperthermophilic bacterium Thermotoga maritima (tmNifS) at 2.0 A resolution. The tmNifS is structured into two domains, the larger bearing the pyridoxal-5'-phosphate-binding active site, the smaller hosting the active site cysteine in the middle of a highly flexible loop, 12 amino acid residues in length. Once charged with sulphur the loop could possibly deliver S(0) directly to regions far remote from the protein. Based on the three-dimensional structures of the native as well as the substrate complexed form and on spectrophotometric results, a mechanism of sulphur activation is proposed. The His99, which stacks on top of the pyridoxal-5'-phosphate co-factor, is assigned a crucial role during the catalytic cycle by acting as an acid-base catalyst and is believed to have a pK(a) value depending on the co-factor redox state.
<StructureSection load='1ecx' size='340' side='right'caption='[[1ecx]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1ecx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ECX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ECX FirstGlance]. <br>
1ECX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with <scene name='pdbligand=PLP:'>PLP</scene> and <scene name='pdbligand=CYS:'>CYS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ECX OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ecx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ecx OCA], [https://pdbe.org/1ecx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ecx RCSB], [https://www.ebi.ac.uk/pdbsum/1ecx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ecx ProSAT]</span></td></tr>
Crystal structure of a NifS-like protein from Thermotoga maritima: implications for iron sulphur cluster assembly., Kaiser JT, Clausen T, Bourenkow GP, Bartunik HD, Steinbacher S, Huber R, J Mol Biol. 2000 Mar 24;297(2):451-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10715213 10715213]
</table>
[[Category: Single protein]]
== Function ==
[https://www.uniprot.org/uniprot/Q9X218_THEMA Q9X218_THEMA]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ec/1ecx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ecx ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: Bartunik, H D.]]
[[Category: Bartunik H-D]]
[[Category: Bourenkow, G P.]]
[[Category: Bourenkow GP]]
[[Category: Clausen, T C.]]
[[Category: Clausen TC]]
[[Category: Huber, R.]]
[[Category: Huber R]]
[[Category: Kaiser, J T.]]
[[Category: Kaiser JT]]
[[Category: Steinbacher, S.]]
[[Category: Steinbacher S]]
[[Category: CYS]]
[[Category: PLP]]
[[Category: c-s beta lyase]]
[[Category: iron-sulfur-cluster synthesis]]
[[Category: plp-dependent enzymes]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:26:25 2008''

Latest revision as of 10:01, 7 February 2024

NIFS-LIKE PROTEINNIFS-LIKE PROTEIN

Structural highlights

1ecx is a 2 chain structure with sequence from Thermotoga maritima. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.7Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9X218_THEMA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1ecx, resolution 2.70Å

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