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New page: left|200px<br /><applet load="1dvo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dvo, resolution 2.00Å" /> '''THE X-RAY CRYSTAL ST...
 
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'''THE X-RAY CRYSTAL STRUCTURE OF FINO, A REPRESSOR OF BACTERIAL CONJUGATION'''<br />


==Overview==
==THE X-RAY CRYSTAL STRUCTURE OF FINO, A REPRESSOR OF BACTERIAL CONJUGATION==
The conjugative transfer of F-like plasmids is repressed by FinO, an RNA, binding protein. FinO interacts with the F-plasmid encoded traJ mRNA and, its antisense RNA, FinP, stabilizing FinP against endonucleolytic, degradation and facilitating sense-antisense RNA recognition. Here we, present the 2.0 A resolution X-ray crystal structure of FinO, lacking its, flexible N-terminal extension. FinO adopts a novel, elongated, largely, helical conformation. An N-terminal region, previously shown to contact, RNA, forms a positively charged alpha-helix (helix 1) that protrudes 45 A, from the central core of FinO. A C-terminal region of FinO that is, implicated in RNA interactions also extends out from the central body of, the protein, adopting a helical conformation and packing against the base, of the N-terminal helix. A highly positively charged patch on the surface, of the FinO core may present another RNA binding surface. The results of, an in vitro RNA duplexing assay demonstrate that the flexible N-terminal, region of FinO plays a key role in FinP-traJ RNA recognition, and supports, our proposal that this region and the N-terminus of helix 1 interact with, and stabilize paired, complementary RNA loops in a kissing complex.
<StructureSection load='1dvo' size='340' side='right'caption='[[1dvo]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1dvo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DVO FirstGlance]. <br>
1DVO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DVO OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dvo OCA], [https://pdbe.org/1dvo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dvo RCSB], [https://www.ebi.ac.uk/pdbsum/1dvo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dvo ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of the bacterial conjugation repressor finO., Ghetu AF, Gubbins MJ, Frost LS, Glover JN, Nat Struct Biol. 2000 Jul;7(7):565-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10876242 10876242]
== Function ==
[https://www.uniprot.org/uniprot/FINO3_ECOLX FINO3_ECOLX] One of the components on the FinOP fertility inhibition complex, which inhibits the expression of traJ gene, which in turn regulates the expression of some 20 transfer genes. The transfer genes are responsible for the process, called conjugal transfer, in which DNA is transmitted from one bacterial host to another. RNA-binding that interacts with the traJ mRNA and its antisense RNA, finP, stabilizing finP against endonucleolytic degradation and facilitating sense-antisense RNA recognition (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dv/1dvo_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dvo ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Frost, L.S.]]
[[Category: Frost LS]]
[[Category: Ghetu, A.F.]]
[[Category: Ghetu AF]]
[[Category: Glover, J.N.M.]]
[[Category: Glover JNM]]
[[Category: Gubbins, M.J.]]
[[Category: Gubbins MJ]]
[[Category: bacterial conjugation]]
[[Category: repressor]]
[[Category: x-ray crystallography]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:37:31 2007''

Latest revision as of 09:59, 7 February 2024

THE X-RAY CRYSTAL STRUCTURE OF FINO, A REPRESSOR OF BACTERIAL CONJUGATIONTHE X-RAY CRYSTAL STRUCTURE OF FINO, A REPRESSOR OF BACTERIAL CONJUGATION

Structural highlights

1dvo is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FINO3_ECOLX One of the components on the FinOP fertility inhibition complex, which inhibits the expression of traJ gene, which in turn regulates the expression of some 20 transfer genes. The transfer genes are responsible for the process, called conjugal transfer, in which DNA is transmitted from one bacterial host to another. RNA-binding that interacts with the traJ mRNA and its antisense RNA, finP, stabilizing finP against endonucleolytic degradation and facilitating sense-antisense RNA recognition (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1dvo, resolution 2.00Å

Drag the structure with the mouse to rotate

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