1dsl: Difference between revisions

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New page: left|200px<br /><applet load="1dsl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dsl, resolution 1.55Å" /> '''GAMMA B CRYSTALLIN C...
 
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[[Image:1dsl.gif|left|200px]]<br /><applet load="1dsl" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1dsl, resolution 1.55&Aring;" />
'''GAMMA B CRYSTALLIN C-TERMINAL DOMAIN'''<br />


==Overview==
==GAMMA B CRYSTALLIN C-TERMINAL DOMAIN==
We use protein engineering and crystallography to simulate aspects of the, early evolution of beta gamma-crystallins by observing how a single domain, oligomerizes in response to changes in a sequence extension. The crystal, structure of the C-terminal domain of gamma beta-crystallin with its, four-residue C-terminal extension shows that the domain does not form a, symmetric homodimer analogous to the two-domain pairing in beta, gamma-crystallins. Instead the C-terminal extension now forms heterologous, interactions with other domains leading to the solvent exposure of the, natural hydrophobic interface with a consequent loss in protein, solubility. However, this domain truncated by just the C-terminal tyrosine, forms a symmetric homodimer of domains in the crystal lattice.
<StructureSection load='1dsl' size='340' side='right'caption='[[1dsl]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dsl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DSL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dsl OCA], [https://pdbe.org/1dsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dsl RCSB], [https://www.ebi.ac.uk/pdbsum/1dsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dsl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CRGB_BOVIN CRGB_BOVIN] Crystallins are the dominant structural components of the vertebrate eye lens.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ds/1dsl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dsl ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1DSL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DSL OCA].
*[[Crystallin 3D structures|Crystallin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The X-ray structures of two mutant crystallin domains shed light on the evolution of multi-domain proteins., Norledge BV, Mayr EM, Glockshuber R, Bateman OA, Slingsby C, Jaenicke R, Driessen HP, Nat Struct Biol. 1996 Mar;3(3):267-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8605629 8605629]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bateman, O.A.]]
[[Category: Bateman OA]]
[[Category: Driessen, H.P.C.]]
[[Category: Driessen HPC]]
[[Category: Glockshuber, R.]]
[[Category: Glockshuber R]]
[[Category: Jaenicke, R.]]
[[Category: Jaenicke R]]
[[Category: Mayr, E.M.]]
[[Category: Mayr E-M]]
[[Category: Norledge, B.V.]]
[[Category: Norledge BV]]
[[Category: Slingsby, C.]]
[[Category: Slingsby C]]
[[Category: eye lens protein]]
[[Category: multigene family]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:32:57 2007''

Latest revision as of 09:58, 7 February 2024

GAMMA B CRYSTALLIN C-TERMINAL DOMAINGAMMA B CRYSTALLIN C-TERMINAL DOMAIN

Structural highlights

1dsl is a 1 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.55Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CRGB_BOVIN Crystallins are the dominant structural components of the vertebrate eye lens.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1dsl, resolution 1.55Å

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