1dpt: Difference between revisions

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New page: left|200px<br /> <applet load="1dpt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dpt, resolution 1.54Å" /> '''D-DOPACHROME TAUTOM...
 
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[[Image:1dpt.gif|left|200px]]<br />
<applet load="1dpt" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1dpt, resolution 1.54&Aring;" />
'''D-DOPACHROME TAUTOMERASE'''<br />


==Overview==
==D-DOPACHROME TAUTOMERASE==
D-Dopachrome tautomerase shares a low homologous amino acid sequence (33%, homology) with the macrophage migration inhibitory factor (MIF) and, possesses similar tautomerase activity as well. MIF is a cytokine involved, in inflammatory reactions and immune responses. Whereas recent studies, have identified MIF as a pituitary hormone and immunoregulator, much less, is known about the structural basis of these physiological functions and, the real significance of tautomerase activity. Therefore, interest in the, structure-function relationship between D-dopachrome tautomerase and MIF, has increased, especially with regard to inflammation and immune, responses. We have determined the X-ray crystal structure of human, D-dopachrome tautomerase at 1.54 A resolution. D-Dopachrome tautomerase, folds to form a homotrimer that has extensive contact between subunits by, intersubunit beta-sheets. Its overall topology and trimeric formations are, similar to those of human MIF. The N-terminal proline is located at the, bottom of a positively charged pocket in which the conformations of Lys32, and Ser63 are highly conserved. These positively charged properties are, also seen in the active site pocket of human MIF, bacterial, 5-(carboxymethyl)-2-hydroxymuconate isomerase (CHMI), and 4-oxalocrotonate, tautomerase (4-OT). A detailed comparison of these structures revealed, significant differences in the environment around the potential active, site, the intersubunit contacts, and charge distribution on the molecular, surface. It can be concluded that these features are related to the, physiological role and tautomerase activity of MIF and D-dopachrome, tautomerase. The present structural study could be helpful for designing, effective inhibitors that modulate immunoregulatory and hormone-like, effects.
<StructureSection load='1dpt' size='340' side='right'caption='[[1dpt]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dpt]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DPT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dpt OCA], [https://pdbe.org/1dpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dpt RCSB], [https://www.ebi.ac.uk/pdbsum/1dpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dpt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DOPD_HUMAN DOPD_HUMAN] Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/1dpt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dpt ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1DPT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DPT OCA].
*[[Macrophage inhibitory factor 3D structures|Macrophage inhibitory factor 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of human D-dopachrome tautomerase, a homologue of macrophage migration inhibitory factor, at 1.54 A resolution., Sugimoto H, Taniguchi M, Nakagawa A, Tanaka I, Suzuki M, Nishihira J, Biochemistry. 1999 Mar 16;38(11):3268-79. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10079069 10079069]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Nakagawa, A.]]
[[Category: Nakagawa A]]
[[Category: Sugimoto, H.]]
[[Category: Sugimoto H]]
[[Category: Tanaka, I.]]
[[Category: Tanaka I]]
[[Category: Taniguchi, M.]]
[[Category: Taniguchi M]]
[[Category: cytokine]]
[[Category: growth factor]]
[[Category: tautomerase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:34:49 2007''

Latest revision as of 09:57, 7 February 2024

D-DOPACHROME TAUTOMERASED-DOPACHROME TAUTOMERASE

Structural highlights

1dpt is a 3 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.54Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DOPD_HUMAN Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1dpt, resolution 1.54Å

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