1dn4: Difference between revisions

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New page: left|200px<br /><applet load="1dn4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dn4, resolution 1.600Å" /> '''SOLVATION OF THE LE...
 
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[[Image:1dn4.gif|left|200px]]<br /><applet load="1dn4" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1dn4, resolution 1.600&Aring;" />
'''SOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURES'''<br />


==Overview==
==SOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURES==
Crystals of the hexadeoxyoligomer d(5BrC-G-5BrC-G-5BrC-G) were grown at, different temperatures (5 degrees C, 18 degrees C and 37 degrees C) in the, absence of divalent cations. The crystals grown at 5 degrees C did not, diffract X-rays, while those grown at 18 degrees C and 37 degrees C did., The oligomer adopts the left-handed ZI conformation in both crystals. The, main difference resides in a more extensive hydration shell in the crystal, grown at high temperature than in the crystal grown at low temperature., The high-temperature crystal displays a spine of hydration running deep in, the minor groove and linking exocyclic O-2 atoms of the pyrimidine rings., In both crystalline forms, a hydrated sodium ion bound to the N-7 of a, guanine ring was found. Strings of water molecules bridging phosphate, anionic oxygen atoms are found along the backbone. The absolute values of, the propeller-twist are also different in both structures although the, values of the twist are very similar. The results point to the importance, of the crystallization conditions when analysing fine structural details, like solvation properties of oligomers.
<StructureSection load='1dn4' size='340' side='right'caption='[[1dn4]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1dn4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DN4 FirstGlance]. <br>
1DN4 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DN4 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CBR:5-BROMO-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>CBR</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dn4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dn4 OCA], [https://pdbe.org/1dn4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dn4 RCSB], [https://www.ebi.ac.uk/pdbsum/1dn4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dn4 ProSAT]</span></td></tr>
Solvation of the left-handed hexamer d(5BrC-G-5BrC-G-5 BrC-G) in crystals grown at two temperatures., Chevrier B, Dock AC, Hartmann B, Leng M, Moras D, Thuong MT, Westhof E, J Mol Biol. 1986 Apr 20;188(4):707-19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3735433 3735433]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Chevrier, B.]]
</StructureSection>
[[Category: Dock, A.C.]]
[[Category: Large Structures]]
[[Category: Hartmann, B.]]
[[Category: Chevrier B]]
[[Category: Leng, M.]]
[[Category: Dock AC]]
[[Category: Moras, D.]]
[[Category: Hartmann B]]
[[Category: Thuong, M.T.]]
[[Category: Leng M]]
[[Category: Westhof, E.]]
[[Category: Moras D]]
[[Category: double helix]]
[[Category: Thuong MT]]
[[Category: modified]]
[[Category: Westhof E]]
[[Category: z-dna]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:20:58 2007''

Latest revision as of 09:56, 7 February 2024

SOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURESSOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURES

Structural highlights

1dn4 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

1dn4, resolution 1.60Å

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