1diw: Difference between revisions

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New page: left|200px<br /><applet load="1diw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1diw, resolution 2.0Å" /> '''THE HC FRAGMENT OF TE...
 
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[[Image:1diw.gif|left|200px]]<br /><applet load="1diw" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1diw, resolution 2.0&Aring;" />
'''THE HC FRAGMENT OF TETANUS TOXIN COMPLEXED WITH GALACTOSE'''<br />


==Overview==
==THE HC FRAGMENT OF TETANUS TOXIN COMPLEXED WITH GALACTOSE==
The entry of tetanus neurotoxin into neuronal cells proceeds through the, initial binding of the toxin to gangliosides on the cell surface. The, carboxyl-terminal fragment of the heavy chain of tetanus neurotoxin, contains the ganglioside-binding site, which has not yet been fully, characterized. The crystal structures of native H(C) and of H(C) soaked, with carbohydrates reveal a number of binding sites and provide insight, into the possible mode of ganglioside binding.
<StructureSection load='1diw' size='340' side='right'caption='[[1diw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1diw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DIW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DIW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1diw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1diw OCA], [https://pdbe.org/1diw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1diw RCSB], [https://www.ebi.ac.uk/pdbsum/1diw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1diw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TETX_CLOTE TETX_CLOTE] Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/di/1diw_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1diw ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1DIW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_tetani Clostridium tetani] with GAL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Tentoxilysin Tentoxilysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.68 3.4.24.68] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DIW OCA].
*[[Tetanus toxin|Tetanus toxin]]
 
__TOC__
==Reference==
</StructureSection>
The structures of the H(C) fragment of tetanus toxin with carbohydrate subunit complexes provide insight into ganglioside binding., Emsley P, Fotinou C, Black I, Fairweather NF, Charles IG, Watts C, Hewitt E, Isaacs NW, J Biol Chem. 2000 Mar 24;275(12):8889-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10722735 10722735]
[[Category: Clostridium tetani]]
[[Category: Clostridium tetani]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Tentoxilysin]]
[[Category: Black I]]
[[Category: Black, I.]]
[[Category: Charles IG]]
[[Category: Charles, I.G.]]
[[Category: Emsley P]]
[[Category: Emsley, P.]]
[[Category: Fairweather NF]]
[[Category: Fairweather, N.F.]]
[[Category: Fotinou C]]
[[Category: Fotinou, C.]]
[[Category: Hewitt E]]
[[Category: Hewitt, E.]]
[[Category: Isaacs NW]]
[[Category: Isaacs, N.W.]]
[[Category: Watts C]]
[[Category: Watts, C.]]
[[Category: GAL]]
[[Category: beta trefoil]]
[[Category: jelly-roll]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:18:49 2007''

Latest revision as of 09:54, 7 February 2024

THE HC FRAGMENT OF TETANUS TOXIN COMPLEXED WITH GALACTOSETHE HC FRAGMENT OF TETANUS TOXIN COMPLEXED WITH GALACTOSE

Structural highlights

1diw is a 1 chain structure with sequence from Clostridium tetani. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TETX_CLOTE Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1diw, resolution 2.00Å

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