1dfu: Difference between revisions

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New page: left|200px<br /><applet load="1dfu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dfu, resolution 1.8Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1dfu.gif|left|200px]]<br /><applet load="1dfu" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1dfu, resolution 1.8&Aring;" />
'''CRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTION'''<br />


==Overview==
==CRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTION==
The crystal structure of Escherichia coli ribosomal protein L25 bound to, an 18-base pair portion of 5S ribosomal RNA, which contains "loop E," has, been determined at 1.8-A resolution. The protein primarily recognizes a, unique RNA shape, although five side chains make direct or water-mediated, interactions with bases. Three beta-strands lie in the widened minor, groove of loop E formed by noncanonical base pairs and cross-strand purine, stacks, and an alpha-helix interacts in an adjacent widened major groove., The structure of loop E is largely the same as that of uncomplexed RNA, (rms deviation of 0.4 A for 11 base pairs), and 3 Mg(2+) ions that, stabilize the noncanonical base pairs lie in the same or similar locations, in both structures. Perhaps surprisingly, those residues interacting with, the RNA backbone are the most conserved among known L25 sequences, whereas, those interacting with the bases are not.
<StructureSection load='1dfu' size='340' side='right'caption='[[1dfu]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dfu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DFU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dfu OCA], [https://pdbe.org/1dfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dfu RCSB], [https://www.ebi.ac.uk/pdbsum/1dfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dfu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL25_ECOLI RL25_ECOLI] This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance. Binds to the 5S rRNA independently of L5 and L18. Not required for binding of the 5S rRNA/L5/L18 subcomplex to 23S rRNA.[HAMAP-Rule:MF_01336]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/df/1dfu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dfu ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1DFU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DFU OCA].
*[[Ribosomal protein L25|Ribosomal protein L25]]
 
__TOC__
==Reference==
</StructureSection>
Structure of Escherichia coli ribosomal protein L25 complexed with a 5S rRNA fragment at 1.8-A resolution., Lu M, Steitz TA, Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2023-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10696113 10696113]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Lu, M.]]
[[Category: Lu M]]
[[Category: Steitz, T.A.]]
[[Category: Steitz TA]]
[[Category: MG]]
[[Category: protein-rna complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:15:23 2007''

Latest revision as of 09:53, 7 February 2024

CRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTIONCRYSTAL STRUCTURE OF E.COLI RIBOSOMAL PROTEIN L25 COMPLEXED WITH A 5S RRNA FRAGMENT AT 1.8 A RESOLUTION

Structural highlights

1dfu is a 3 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RL25_ECOLI This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance. Binds to the 5S rRNA independently of L5 and L18. Not required for binding of the 5S rRNA/L5/L18 subcomplex to 23S rRNA.[HAMAP-Rule:MF_01336]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1dfu, resolution 1.80Å

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