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| [[Image:1dcv.gif|left|200px]]<br /><applet load="1dcv" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1dcv, resolution 2.50Å" />
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| '''B-DNA DECAMER WITH CENTRAL TA DINUCLEOTIDE'''<br />
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| ==Overview== | | ==B-DNA DECAMER WITH CENTRAL TA DINUCLEOTIDE== |
| Holliday junctions are important structural intermediates in recombination, viral integration, and DNA repair. We present here the single-crystal structure of the inverted repeat sequence d(CCGGTACCGG) as a Holliday junction at the nominal resolution of 2. 1 A. Unlike the previous crystal structures, this DNA junction has B-DNA arms with all standard Watson-Crick base pairs; it therefore represents the intermediate proposed by Holliday as being involved in homologous recombination. The junction is in the stacked-X conformation, with two interconnected duplexes formed by coaxially stacked arms, and is crossed at an angle of 41.4 degrees as a right-handed X. A sequence comparison with previous B-DNA and junction crystal structures shows that an ACC trinucleotide forms the core of a stable junction in this system. The 3'-C x G base pair of this ACC core forms direct and water-mediated hydrogen bonds to the phosphates at the crossover strands. Interactions within this core define the conformation of the Holliday junction, including the angle relating the stacked duplexes and how the base pairs are stacked in the stable form of the junction.
| | <StructureSection load='1dcv' size='340' side='right'caption='[[1dcv]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[1dcv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DCV FirstGlance]. <br> |
| 1DCV is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCV OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dcv OCA], [https://pdbe.org/1dcv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dcv RCSB], [https://www.ebi.ac.uk/pdbsum/1dcv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dcv ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| The Holliday junction in an inverted repeat DNA sequence: sequence effects on the structure of four-way junctions., Eichman BF, Vargason JM, Mooers BH, Ho PS, Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):3971-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10760268 10760268]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Eichman, B F.]] | | [[Category: Large Structures]] |
| [[Category: Ho, P S.]] | | [[Category: Eichman BF]] |
| [[Category: Mooers, B H.M.]] | | [[Category: Ho PS]] |
| [[Category: Vargason, J M.]] | | [[Category: Mooers BHM]] |
| [[Category: central ta dinucleotide]] | | [[Category: Vargason JM]] |
| [[Category: deoxyribonucleic acid]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:21 2008''
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