1d9v: Difference between revisions

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New page: left|200px<br /><applet load="1d9v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d9v, resolution 1.75Å" /> '''HAEMOPHILUS INFLUENZ...
 
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[[Image:1d9v.jpg|left|200px]]<br /><applet load="1d9v" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1d9v, resolution 1.75&Aring;" />
'''HAEMOPHILUS INFLUENZAE FERRIC-BINDING PROTEIN APO FORM'''<br />


==Overview==
==HAEMOPHILUS INFLUENZAE FERRIC-BINDING PROTEIN APO FORM==
The crystal structure of the iron-free (apo) form of the Haemophilus, influenzae Fe(3+)-binding protein (hFbp) has been determined to 1.75 A, resolution. Information from this structure complements that derived from, the holo structure with respect to the delineation of the process of iron, binding and release. A 21 degrees rotation separates the two structural, domains when the apo form is compared with the holo conformer, indicating, that upon release of iron, the protein undergoes a change in conformation, by bending about the central beta-sheet hinge. A surprising finding in the, apo-hFbp structure was that the ternary binding site anion, observed in, the crystals as phosphate, remained bound. In solution, apo-hFbp bound, phosphate with an affinity K(d) of 2.3 x 10(-3) M. The presence of this, ternary binding site anion appears to arrange the C-terminal iron-binding, residues conducive to complementary binding to Fe(3+), while residues in, the N-terminal binding domain must undergo induced fit to accommodate the, Fe(3+) ligand. These observations suggest a binding process, the first, step of which is the binding of a synergistic anion such as phosphate to, the C-terminal domain. Next, iron binds to the preordered half-site on the, C-terminal domain. Finally, the presence of iron organizes the N-terminal, half-site and closes the interdomain hinge. The use of the synergistic, anion and this iron binding process results in an extremely high affinity, of the Fe(3+)-binding proteins for Fe(3+) (nFbp K'(eff) = 2.4 x 10(18), M(-1)). This high-affinity ligand binding process is unique among the, family of bacterial periplasmic binding proteins and has interesting, implications in the mechanism of iron removal from the Fe(3+)-binding, proteins during FbpABC-mediated iron transport across the cytoplasmic, membrane.
<StructureSection load='1d9v' size='340' side='right'caption='[[1d9v]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1d9v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D9V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D9V FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d9v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d9v OCA], [https://pdbe.org/1d9v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d9v RCSB], [https://www.ebi.ac.uk/pdbsum/1d9v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d9v ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FBPA_HAEIN FBPA_HAEIN] Part of the ABC transporter complex FbpABC (TC 3.A.1.10.1) involved in Fe(3+) ions import. This protein specifically binds Fe(3+) and is involved in its transmembrane transport (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d9/1d9v_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d9v ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1D9V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D9V OCA].
*[[Ferric-binding protein|Ferric-binding protein]]
 
__TOC__
==Reference==
</StructureSection>
Crystallographic and biochemical analyses of the metal-free Haemophilus influenzae Fe3+-binding protein., Bruns CM, Anderson DS, Vaughan KG, Williams PA, Nowalk AJ, McRee DE, Mietzner TA, Biochemistry. 2001 Dec 25;40(51):15631-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11747438 11747438]
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bruns, C.M.]]
[[Category: Bruns CM]]
[[Category: McRee, D.E.]]
[[Category: McRee DE]]
[[Category: Williams, P.A.]]
[[Category: Williams PA]]
[[Category: PO4]]
[[Category: abc cassette receptor protein]]
[[Category: apo form]]
[[Category: binding protein]]
[[Category: ferric]]
[[Category: iron]]
[[Category: periplasmic protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:06:46 2007''

Latest revision as of 09:51, 7 February 2024

HAEMOPHILUS INFLUENZAE FERRIC-BINDING PROTEIN APO FORMHAEMOPHILUS INFLUENZAE FERRIC-BINDING PROTEIN APO FORM

Structural highlights

1d9v is a 1 chain structure with sequence from Haemophilus influenzae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.75Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FBPA_HAEIN Part of the ABC transporter complex FbpABC (TC 3.A.1.10.1) involved in Fe(3+) ions import. This protein specifically binds Fe(3+) and is involved in its transmembrane transport (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1d9v, resolution 1.75Å

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