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| [[Image:1d99.gif|left|200px]]
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| {{Structure
| | ==STRUCTURAL FEATURES AND HYDRATION OF A DODECAMER DUPLEX CONTAINING TWO C.A MISPAIRS== |
| |PDB= 1d99 |SIZE=350|CAPTION= <scene name='initialview01'>1d99</scene>, resolution 2.500Å
| | <StructureSection load='1d99' size='340' side='right'caption='[[1d99]], [[Resolution|resolution]] 2.50Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>
| | <table><tr><td colspan='2'>[[1d99]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D99 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d99 OCA], [https://pdbe.org/1d99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d99 RCSB], [https://www.ebi.ac.uk/pdbsum/1d99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d99 ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | __TOC__ |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d99 OCA], [http://www.ebi.ac.uk/pdbsum/1d99 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d99 RCSB]</span>
| | </StructureSection> |
| }}
| | [[Category: Large Structures]] |
| | | [[Category: Brown T]] |
| '''STRUCTURAL FEATURES AND HYDRATION OF A DODECAMER DUPLEX CONTAINING TWO C.A MISPAIRS'''
| | [[Category: Hunter WN]] |
| | | [[Category: Kennard O]] |
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| ==Overview==
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| X-ray diffraction techniques have been used to characterise the crystal and molecular structure of the deoxyoligomer d(C-G-C-A-A-A-T-T-C-G-C-G) at 2.5A resolution. The final R factor is 0.19 with the location of 78 solvent molecules. The oligomer crystallises in a B-DNA type conformation with two strands coiled about each other to produce a duplex. This double helix consists of four A.T and six G.C Watson-Crick base pairs and two C.A mispairs. The mismatched base pairs adopt a "wobble" type structure with the cytosine displaced laterally into the major groove, the adenine into the minor groove. We have proposed that the two close contacts observed in the C.A pairing represent two hydrogen bonds one of which results from protonation of adenine. The mispairs are accommodated in the double helix with small adjustments in the conformation of the sugar-phosphate backbone. Details of the backbone conformation, base stacking interactions, thermal parameters and the hydration are now presented and compared with those of the native oligomer d(C-G-C-G-A-A-T-T-C-G-C-G) and with variations of this sequence containing G.T and G.A mispairs.
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| ==About this Structure==
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| 1D99 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D99 OCA].
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| ==Reference==
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| Structural features and hydration of a dodecamer duplex containing two C.A mispairs., Hunter WN, Brown T, Kennard O, Nucleic Acids Res. 1987 Aug 25;15(16):6589-606. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3627999 3627999]
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| [[Category: Protein complex]] | |
| [[Category: Brown, T.]] | |
| [[Category: Hunter, W N.]] | |
| [[Category: Kennard, O.]] | |
| [[Category: b-dna]]
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| [[Category: double helix]]
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| [[Category: mismatched]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:37:04 2008''
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