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| [[Image:1d80.gif|left|200px]]<br /><applet load="1d80" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="1d80, resolution 2.200Å" />
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| '''CRYSTAL STRUCTURE OF AN OLIGONUCLEOTIDE DUPLEX CONTAINING G.G BASE-PAIRS: THE INFLUENCE OF MISPAIRING ON DNA BACKBONE CONFORMATION'''<br />
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| ==Overview== | | ==CRYSTAL STRUCTURE OF AN OLIGONUCLEOTIDE DUPLEX CONTAINING G.G BASE-PAIRS: THE INFLUENCE OF MISPAIRING ON DNA BACKBONE CONFORMATION== |
| The structure of the G.G mispaired dodecanucleotide d(CGCGAATTGGCG)2 has been solved by x-ray crystallography and refined to an R factor of 18.8% at 2.2 A resolution for 3513 reflections. The dodecamer crystallizes as a B-type DNA double helix. It contains two G(anti).G(syn) base pairs--i.e., G-4/G-16(anti).G-21/G-9(syn). The Hoogsteen base pairing involves atoms O-6 and N-7 of the guanine in the syn conformation with atoms N-1 and N-2 of the anti-paired purine. One G.G base pair has a bifurcated hydrogen bond between G-4(N-1)...G-21(N-7) and G-4(N-1)...G-21(O-6). There is little overall structural distortion of the double helix induced as a consequence of the mispairing. The helical width is significantly increased by comparison with the structure of the native duplex, and the minor groove width in the 5'-AATT region is decreased. The G.G base pairing induces high-BII phosphate conformations at residues G-9 and T-20 in addition to more normal BII conformations at G-10 and G-22. It is suggested that these backbone aberrations provide signals for the facile repairability of G.G mispairs in DNA.
| | <StructureSection load='1d80' size='340' side='right'caption='[[1d80]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[1d80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D80 FirstGlance]. <br> |
| 1D80 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D80 OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d80 OCA], [https://pdbe.org/1d80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d80 RCSB], [https://www.ebi.ac.uk/pdbsum/1d80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d80 ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| Crystal structure of an oligonucleotide duplex containing G.G base pairs: influence of mispairing on DNA backbone conformation., Skelly JV, Edwards KJ, Jenkins TC, Neidle S, Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):804-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8430089 8430089]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Edwards, K J.]]
| | [[Category: Large Structures]] |
| [[Category: Jenkins, T C.]]
| | [[Category: Edwards KJ]] |
| [[Category: Neidle, S.]] | | [[Category: Jenkins TC]] |
| [[Category: Skelly, J V.]] | | [[Category: Neidle S]] |
| [[Category: b-dna]] | | [[Category: Skelly JV]] |
| [[Category: double helix]] | |
| [[Category: mismatched]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:00 2008''
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