1d3v: Difference between revisions

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OCA

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-[[Image:1d3v.gif|left|200px]]<br /><applet load="1d3v" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1d3v, resolution 1.70&Aring;" />
-'''CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH 2(S)-AMINO-6-BORONOHEXANOIC ACID, AN L-ARGININE ANALOG'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH 2(S)-AMINO-6-BORONOHEXANOIC ACID, AN L-ARGININE ANALOG==
-The crystal structure of the complex between the binuclear manganese, metalloenzyme arginase and the boronic acid analog of L-arginine, 2(S)-amino-6-boronohexanoic acid (ABH), has been determined at 1.7 A, resolution from a crystal perfectly twinned by hemihedry. ABH binds as the, tetrahedral boronate anion, with one hydroxyl oxygen symmetrically, bridging the binuclear manganese cluster and a second hydroxyl oxygen, coordinating to Mn2+A. This binding mode mimics the transition state of a, metal-activated hydroxide mechanism. This transition state structure, differs from that occurring in NO biosynthesis, thereby explaining why ABH, does not inhibit NO synthase. We also show that arginase activity is, present in the penis. Accordingly, the tight binding and specificity of, ABH allows us to probe the physiological role of arginase in modulating, the NO-dependent smooth muscle relaxation required for erection., Strikingly, ABH causes significant enhancement of nonadrenergic, noncholinergic nerve-mediated relaxation of penile corpus cavernosum, smooth muscle, suggesting that arginase inhibition sustains L-arginine, concentrations for NO synthase activity. Therefore, human penile arginase, is a potential target for therapeutic intervention in the treatment of, erectile dysfunction.
+<StructureSection load='1d3v' size='340' side='right'caption='[[1d3v]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1d3v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D3V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D3V FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABH:2(S)-AMINO-6-BORONOHEXANOIC+ACID'>ABH</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d3v OCA], [https://pdbe.org/1d3v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d3v RCSB], [https://www.ebi.ac.uk/pdbsum/1d3v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d3v ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ARGI1_RAT ARGI1_RAT]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d3/1d3v_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d3v ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-D3V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MN and ABH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Arginase Arginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.1 3.5.3.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D3V OCA].
+*[[Arginase 3D structures|Arginase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Arginase-boronic acid complex highlights a physiological role in erectile function., Cox JD, Kim NN, Traish AM, Christianson DW, Nat Struct Biol. 1999 Nov;6(11):1043-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10542097 10542097]
+[[Category: Large Structures]]
-[[Category: Arginase]]
 [[Category: Rattus norvegicus]]
-[[Category: Single protein]]
+[[Category: Christianson DW]]
-[[Category: Christianson, D.W.]]
+[[Category: Cox JD]]
-[[Category: Cox, J.D.]]
+[[Category: Kim NN]]
-[[Category: Kim, N.N.]]
+[[Category: Traish AM]]
-[[Category: Traish, A.M.]]
-[[Category: ABH]]
-[[Category: MN]]
-[[Category: binuclear manganese cluster]]
-[[Category: boronic acid inhibitor]]
-[[Category: hydrolase]]
-[[Category: perfectly twinned crystal]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:59:38 2007''