1d39: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /><applet load="1d39" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d39, resolution 1.200Å" /> '''COVALENT MODIFICATI...
 
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1d39.gif|left|200px]]<br /><applet load="1d39" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1d39, resolution 1.200&Aring;" />
'''COVALENT MODIFICATION OF GUANINE BASES IN DOUBLE STRANDED DNA: THE 1.2 ANGSTROMS Z-DNA STRUCTURE OF D(CGCGCG) IN THE PRESENCE OF CUCL2'''<br />


==Overview==
==COVALENT MODIFICATION OF GUANINE BASES IN DOUBLE STRANDED DNA: THE 1.2 ANGSTROMS Z-DNA STRUCTURE OF D(CGCGCG) IN THE PRESENCE OF CUCL2==
We have solved the single crystal structure to 1.2-A resolution of the, Z-DNA sequence d(CGCGCG) soaked with copper(II) chloride. This structure, allows us to elucidate the structural properties of copper in a model that, mimics a physiologically relevant environment. A copper(II) cation was, observed to form a covalent coordinate bond to N-7 of each guanine base, along the hexamer duplex. The occurrence of copper bound at each site was, dependent on the exposure of the bases and the packing of the hexamers in, the crystal. The copper at the highest occupied site was observed to form, a regular octahedral complex, with four water ligands in the equatorial, plane and a fifth water along with N-7 of the purine base at the axial, positions. All other copper complexes appear to be variations of this, structure. By using the octahedral complex as the prototype for copper(II), binding to guanine bases in the Z-DNA crystal, model structures were built, showing that duplex B-DNA can accommodate octahedral copper(II) complexes, at the guanine bases as well as copper complexes bridged at adjacent, guanine residues by a reactive dioxygen species. The increased, susceptibility to oxidative DNA cleavage induced by copper(II) ions in, solution of the bases located 5' to one or more adjacent guanine residues, can thus be explained in terms of the cation and DNA structures described, by these models.
<StructureSection load='1d39' size='340' side='right'caption='[[1d39]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1d39]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D39 FirstGlance]. <br>
1D39 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with CU and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D39 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d39 OCA], [https://pdbe.org/1d39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d39 RCSB], [https://www.ebi.ac.uk/pdbsum/1d39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d39 ProSAT]</span></td></tr>
Covalent modification of guanine bases in double-stranded DNA. The 1.2-A Z-DNA structure of d(CGCGCG) in the presence of CuCl2., Kagawa TF, Geierstanger BH, Wang AH, Ho PS, J Biol Chem. 1991 Oct 25;266(30):20175-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1939078 1939078]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Geierstanger, B.H.]]
</StructureSection>
[[Category: Ho, P.S.]]
[[Category: Large Structures]]
[[Category: Kagawa, T.F.]]
[[Category: Geierstanger BH]]
[[Category: Wang, A.H.J.]]
[[Category: Ho PS]]
[[Category: CU]]
[[Category: Kagawa TF]]
[[Category: NA]]
[[Category: Wang AH-J]]
[[Category: double helix]]
[[Category: modified]]
[[Category: z-dna]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:23:42 2007''

Latest revision as of 09:48, 7 February 2024

COVALENT MODIFICATION OF GUANINE BASES IN DOUBLE STRANDED DNA: THE 1.2 ANGSTROMS Z-DNA STRUCTURE OF D(CGCGCG) IN THE PRESENCE OF CUCL2COVALENT MODIFICATION OF GUANINE BASES IN DOUBLE STRANDED DNA: THE 1.2 ANGSTROMS Z-DNA STRUCTURE OF D(CGCGCG) IN THE PRESENCE OF CUCL2

Structural highlights

1d39 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

1d39, resolution 1.20Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1d39&oldid=4041544"