1d2k: Difference between revisions

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[[Image:1d2k.gif|left|200px]]


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==C. IMMITIS CHITINASE 1 AT 2.2 ANGSTROMS RESOLUTION==
The line below this paragraph, containing "STRUCTURE_1d2k", creates the "Structure Box" on the page.
<StructureSection load='1d2k' size='340' side='right'caption='[[1d2k]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1d2k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D2K FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d2k OCA], [https://pdbe.org/1d2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d2k RCSB], [https://www.ebi.ac.uk/pdbsum/1d2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d2k ProSAT]</span></td></tr>
{{STRUCTURE_1d2k|  PDB=1d2k |  SCENE= }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/CHI1_COCPS CHI1_COCPS]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d2/1d2k_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d2k ConSurf].
<div style="clear:both"></div>


'''C. IMMITIS CHITINASE 1 AT 2.2 ANGSTROMS RESOLUTION'''
==See Also==
 
*[[Chitinase 3D structures|Chitinase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The X-ray structure of chitinase from the fungal pathogen Coccidioides immitis has been solved to 2.2 A resolution. Like other members of the class 18 hydrolase family, this 427 residue protein is an eight-stranded beta/alpha-barrel. Although lacking an N-terminal chitin anchoring domain, the enzyme closely resembles the chitinase from Serratia marcescens. Among the conserved features are three cis peptide bonds, all involving conserved active site residues. The active site is formed from conserved residues such as tryptophans 47, 131, 315, 378, tyrosines 239 and 293, and arginines 52 and 295. Glu171 is the catalytic acid in the hydrolytic mechanism; it was mutated to a Gln, and activity was abolished. Allosamidin is a substrate analog that strongly inhibits the class 18 enzymes. Its binding to the chitinase hevamine has been observed, and we used conserved structural features of the two enzymes to predict the inhibitors binding to the fungal enzyme.
 
==About this Structure==
1D2K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Coccidioides_immitis Coccidioides immitis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D2K OCA].
 
==Reference==
The X-ray structure of a chitinase from the pathogenic fungus Coccidioides immitis., Hollis T, Monzingo AF, Bortone K, Ernst S, Cox R, Robertus JD, Protein Sci. 2000 Mar;9(3):544-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10752616 10752616]
[[Category: Chitinase]]
[[Category: Coccidioides immitis]]
[[Category: Coccidioides immitis]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bortone, K.]]
[[Category: Bortone K]]
[[Category: Cox, R.]]
[[Category: Cox R]]
[[Category: Ernst, S R.]]
[[Category: Ernst SR]]
[[Category: Hollis, T.]]
[[Category: Hollis T]]
[[Category: Monzingo, A F.]]
[[Category: Monzingo AF]]
[[Category: Robertus, J D.]]
[[Category: Robertus JD]]
[[Category: Beta-alpha barrel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:22:35 2008''

Latest revision as of 09:48, 7 February 2024

C. IMMITIS CHITINASE 1 AT 2.2 ANGSTROMS RESOLUTIONC. IMMITIS CHITINASE 1 AT 2.2 ANGSTROMS RESOLUTION

Structural highlights

1d2k is a 1 chain structure with sequence from Coccidioides immitis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CHI1_COCPS

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1d2k, resolution 2.20Å

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