1d29: Difference between revisions

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[[Image:1d29.gif|left|200px]]


{{Structure
==CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG==
|PDB= 1d29 |SIZE=350|CAPTION= <scene name='initialview01'>1d29</scene>, resolution 2.500&Aring;
<StructureSection load='1d29' size='340' side='right'caption='[[1d29]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
<table><tr><td colspan='2'>[[1d29]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D29 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D29 FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
|GENE=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d29 OCA], [https://pdbe.org/1d29 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d29 RCSB], [https://www.ebi.ac.uk/pdbsum/1d29 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d29 ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=
__TOC__
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d29 OCA], [http://www.ebi.ac.uk/pdbsum/1d29 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d29 RCSB]</span>
</StructureSection>
}}
[[Category: Large Structures]]
 
[[Category: Dickerson RE]]
'''CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG'''
[[Category: Kopka ML]]
 
[[Category: Larsen TA]]
 
==Overview==
The crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has been determined at a resolution of 2.5 A, with a final R factor of 15.8% for 1475 nonzero reflections measured at 0 degrees C. The structure is isomorphous with that of the Drew dodecamer, with the space group P2(1)2(1)2(1) and cell dimensions of a = 24.94 A, b = 40.78 A, and c = 66.13 A. The asymmetric unit contains all 12 base pairs of the B-DNA double helix and 36 water molecules. The structure of C-G-T-G-A-A-T-T-C-A-C-G is very similar to that of C-G-C-G-A-A-T-T-C-G-C-G, with no major alterations in helix parameters. Water peaks in the refined structure appear to represent a selection of peaks that were observed in the Drew dodecamer. The minor-groove spine of hydration at 2.5 A is fragmentary, but as Narendra et al. (1991) [Biochemistry (following paper in this issue)] have observed, lowering the temperature leads to a more complete representation of the spine.
 
==About this Structure==
1D29 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D29 OCA].
 
==Reference==
Crystal structure analysis of the B-DNA dodecamer CGTGAATTCACG., Larsen TA, Kopka ML, Dickerson RE, Biochemistry. 1991 May 7;30(18):4443-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1850624 1850624]
[[Category: Protein complex]]
[[Category: Dickerson, R E.]]
[[Category: Kopka, M L.]]
[[Category: Larsen, T A.]]
[[Category: b-dna]]
[[Category: double helix]]
 
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