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==HIGH-RESOLUTION STRUCTURE OF A MUTAGENIC LESION IN DNA==
==HIGH-RESOLUTION STRUCTURE OF A MUTAGENIC LESION IN DNA==
<StructureSection load='1d27' size='340' side='right' caption='[[1d27]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='1d27' size='340' side='right'caption='[[1d27]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1d27]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D27 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D27 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1d27]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D27 FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6OG:6-O-METHYL+GUANOSINE-5-MONOPHOSPHATE'>6OG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d27 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1d27 RCSB], [http://www.ebi.ac.uk/pdbsum/1d27 PDBsum]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6OG:6-O-METHYL+GUANOSINE-5-MONOPHOSPHATE'>6OG</scene></td></tr>
<table>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d27 OCA], [https://pdbe.org/1d27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d27 RCSB], [https://www.ebi.ac.uk/pdbsum/1d27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d27 ProSAT]</span></td></tr>
<div style="background-color:#fffaf0;">
</table>
== Publication Abstract from PubMed ==
The self-complementary dodecanucleotide d[CGC(m6G)AATTTGCG]2 (where m6G is O6-methylguanine), which contains two m6G.T base pairs, has been analyzed by x-ray diffraction methods and the structure has been refined to a residual error of R = 0.185 at 2.0-A resolution. The m6G.T mispair closely resembles a Watson-Crick base pair and there are very few structural differences between the m6G.T duplex and the native analogue. The similarity between the m6G.T base pair and a normal G.C base pair explains the failure of mismatch repair enzymes to recognize and remove this mutagenic lesion. A series of ultraviolet melting studies over a wide pH range on a related dodecamer indicate that the m6G.C mispair can exist in two conformations; one is a wobble pair and the other is a protonated Watson-Crick pair. The former, which predominates at physiological pH, will be removed by normal proofreading and repair enzymes, whereas the latter is likely to escape detection. Hence, the occasional occurrence of the protonated m6G.C base pair may explain why the presence of m6G in genomic DNA does not always give rise to a mutation.
 
High-resolution structure of a mutagenic lesion in DNA.,Leonard GA, Thomson J, Watson WP, Brown T Proc Natl Acad Sci U S A. 1990 Dec;87(24):9573-6. PMID:2263612<ref>PMID:2263612</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Brown, T.]]
[[Category: Large Structures]]
[[Category: Leonard, G A.]]
[[Category: Brown T]]
[[Category: Thomson, J.]]
[[Category: Leonard GA]]
[[Category: Watson, W P.]]
[[Category: Thomson J]]
[[Category: B-dna]]
[[Category: Watson WP]]
[[Category: Dna]]
[[Category: Double helix]]
[[Category: Mismatched]]
[[Category: Modified]]

Latest revision as of 09:48, 7 February 2024

HIGH-RESOLUTION STRUCTURE OF A MUTAGENIC LESION IN DNAHIGH-RESOLUTION STRUCTURE OF A MUTAGENIC LESION IN DNA

Structural highlights

1d27 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

1d27, resolution 2.00Å

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OCA
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