1cvm: Difference between revisions

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New page: left|200px<br /><applet load="1cvm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cvm, resolution 2.40Å" /> '''CADMIUM INHIBITED CR...
 
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[[Image:1cvm.gif|left|200px]]<br /><applet load="1cvm" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cvm, resolution 2.40&Aring;" />
'''CADMIUM INHIBITED CRYSTAL STRUCTURE OF PHYTASE FROM BACILLUS AMYLOLIQUEFACIENS'''<br />


==Overview==
==CADMIUM INHIBITED CRYSTAL STRUCTURE OF PHYTASE FROM BACILLUS AMYLOLIQUEFACIENS==
A novel bacterial phytase from a Bacillus amyloliquefaciens strain was, crystallized using the hanging-drop vapour-diffusion method. The, amino-acid sequence of the enzyme does not show any homology to those of, other known phytases or phosphatases, with the exception of a phytase from, Bacillus subtilis. The enzyme exhibits a thermal stability which is, strongly dependent on calcium ions. High-quality single crystals of the, enzyme in the absence of calcium ions were obtained using a precipitant, solution containing 20% 2-methyl-2, 4-pentanediol and 0.1 M MES (pH 6.5)., Native diffraction data to 2.0 A resolution were obtained from a, flash-frozen crystal at 110 K using a rotating-anode X-ray source. The, crystals belong to space group P212121 with unit-cell dimensions a = 50.4, b = 64.1, c = 104. 2 A and contain one monomer per asymmetric unit., Structure determination using heavy-atom derivative crystals is in, progress, along with an effort to crystallize the calcium ion bound form, of the enzyme.
<StructureSection load='1cvm' size='340' side='right'caption='[[1cvm]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cvm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CVM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cvm OCA], [https://pdbe.org/1cvm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cvm RCSB], [https://www.ebi.ac.uk/pdbsum/1cvm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cvm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PHYT_BACSD PHYT_BACSD]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cv/1cvm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cvm ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1CVM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens Bacillus amyloliquefaciens] with CA and CD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CVM OCA].
*[[Phytase 3D structures|Phytase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Preliminary X-ray crystallographic analysis of a novel phytase from a Bacillus amyloliquefaciens strain., Ha NC, Kim YO, Oh TK, Oh BH, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):691-3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089471 10089471]
[[Category: 3-phytase]]
[[Category: Bacillus amyloliquefaciens]]
[[Category: Bacillus amyloliquefaciens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Ha, N.C.]]
[[Category: Ha N-C]]
[[Category: Oh, B.H.]]
[[Category: Oh B-H]]
[[Category: Shin, S.]]
[[Category: Shin S]]
[[Category: CA]]
[[Category: CD]]
[[Category: thermostable bacillus phytase phytate phosphatase cadmium calcium]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:47:20 2007''

Latest revision as of 09:46, 7 February 2024

CADMIUM INHIBITED CRYSTAL STRUCTURE OF PHYTASE FROM BACILLUS AMYLOLIQUEFACIENSCADMIUM INHIBITED CRYSTAL STRUCTURE OF PHYTASE FROM BACILLUS AMYLOLIQUEFACIENS

Structural highlights

1cvm is a 1 chain structure with sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PHYT_BACSD

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1cvm, resolution 2.40Å

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