1cqt: Difference between revisions

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New page: left|200px<br /> <applet load="1cqt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cqt, resolution 3.20Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:1cqt.gif|left|200px]]<br />
<applet load="1cqt" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cqt, resolution 3.20&Aring;" />
'''CRYSTAL STRUCTURE OF A TERNARY COMPLEX CONTAINING AN OCA-B PEPTIDE, THE OCT-1 POU DOMAIN, AND AN OCTAMER ELEMENT'''<br />


==Overview==
==CRYSTAL STRUCTURE OF A TERNARY COMPLEX CONTAINING AN OCA-B PEPTIDE, THE OCT-1 POU DOMAIN, AND AN OCTAMER ELEMENT==
We have determined the crystal structure, at 3.2 A, of a ternary complex, containing an OCA-B peptide, the Oct-1 POU domain, and an octamer DNA, site. The OCA-B peptide binds in the major groove near the center of the, octamer site, and its polypeptide backbone forms a pair of hydrogen bonds, with the adenine base at position 5 of the octamer DNA. Numerous, protein-protein contacts between the OCA-B peptide and the POU domain are, also involved in the ternary complex. In particular, the hydrophobic, surface from a short alpha-helix of OCA-B helps to stabilize the complex, by binding to a hydrophobic pocket on the POU-specific domain. The, structure of this ternary complex is consistent with previous biochemical, studies and shows how peptide-DNA and peptide-protein contacts from OCA-B, provide structural and functional specificity in the regulation of, immunoglobulin transcription.
<StructureSection load='1cqt' size='340' side='right'caption='[[1cqt]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1cqt]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CQT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CQT FirstGlance]. <br>
1CQT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CQT OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cqt OCA], [https://pdbe.org/1cqt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cqt RCSB], [https://www.ebi.ac.uk/pdbsum/1cqt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cqt ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface., Chasman D, Cepek K, Sharp PA, Pabo CO, Genes Dev. 1999 Oct 15;13(20):2650-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10541551 10541551]
== Function ==
[https://www.uniprot.org/uniprot/PO2F1_HUMAN PO2F1_HUMAN] Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.<ref>PMID:1684878</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cq/1cqt_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cqt ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Cepek, K.]]
[[Category: Cepek K]]
[[Category: Chasman, D.I.]]
[[Category: Chasman DI]]
[[Category: Pabo, C.O.]]
[[Category: Pabo CO]]
[[Category: Sharp, P.A.]]
[[Category: Sharp PA]]
[[Category: gene regulation/dna]]
[[Category: oca-b]]
[[Category: oct1]]
[[Category: pou domain]]
[[Category: protein-dna complex]]
[[Category: protein-dna interface]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 16:24:36 2007''

Latest revision as of 09:44, 7 February 2024

CRYSTAL STRUCTURE OF A TERNARY COMPLEX CONTAINING AN OCA-B PEPTIDE, THE OCT-1 POU DOMAIN, AND AN OCTAMER ELEMENTCRYSTAL STRUCTURE OF A TERNARY COMPLEX CONTAINING AN OCA-B PEPTIDE, THE OCT-1 POU DOMAIN, AND AN OCTAMER ELEMENT

Structural highlights

1cqt is a 8 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.2Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PO2F1_HUMAN Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Segil N, Roberts SB, Heintz N. Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activity. Science. 1991 Dec 20;254(5039):1814-6. PMID:1684878

1cqt, resolution 3.20Å

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