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[[Image:1cbq.jpg|left|200px]]<br /><applet load="1cbq" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1cbq, resolution 2.2&Aring;" />
'''CRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOID'''<br />


==Overview==
==CRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOID==
BACKGROUND: Retinoic acid (RA) plays a fundamental role in diverse, cellular activities. Cellular RA binding proteins (CRABPs) are thought to, act by modulating the amount of RA available to nuclear RA receptors., CRABPs and cellular retinol-binding proteins (CRBPs) share a unique fold, of two orthogonal beta-sheets that encapsulate their ligands. It has been, suggested that a trio of residues are the prime determinants defining the, high specificity of CRBPs and CRABPs for their physiological ligands., RESULTS: Bovine/murine CRABP I and human CRABP II have been crystallized, in complex with their natural ligand, all-trans-RA. Human CRABP II has, also been crystallized in complex with a synthetic retinoid, 'compound, 19'. Their structures have been determined and refined at resolutions of, 2.9 A, 1.8 A and 2.2 A, respectively. CONCLUSIONS: The retinoid-binding, site in CRABPs differs significantly from that observed in CRBP., Structural changes in three juxtaposed areas of the protein create a new, displaced binding site for RA. The carboxylate of the ligand interacts, with the expected trio of residues (Arg132, Tyr134 and Arg111; CRABP II, numbering). The RA ligand is almost flat with the beta-ionone ring showing, a significant deviation (-33 degrees) from a cis conformation relative to, the isoprene tail. The edge atoms of the beta-ionone ring are accessible, to solvent in a suitable orientation for presentation to metabolizing, enzymes. The bulkier synthetic retinoid causes small conformational, changes in the protein structure.
<StructureSection load='1cbq' size='340' side='right'caption='[[1cbq]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cbq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CBQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=RE9:6-(2,3,4,5,6,7-HEXAHYDRO-2,4,4-TRIMETHYL-1-METYLENEINDEN-2-YL)-3-METHYLHEXA-2,4-DIENOIC+ACID'>RE9</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cbq OCA], [https://pdbe.org/1cbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cbq RCSB], [https://www.ebi.ac.uk/pdbsum/1cbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cbq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RABP2_HUMAN RABP2_HUMAN] Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cb/1cbq_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cbq ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1CBQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=RE9:'>RE9</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CBQ OCA].
*[[CRABP I ( Cellular Retinoic Acid Binding Protein )|CRABP I ( Cellular Retinoic Acid Binding Protein )]]
 
*[[Cellular retinoic acid-binding protein 3D structures|Cellular retinoic acid-binding protein 3D structures]]
==Reference==
*[[Gustavo Elberto Epalza Sanchez/Sandbox 1|Gustavo Elberto Epalza Sanchez/Sandbox 1]]
Crystal structures of cellular retinoic acid binding proteins I and II in complex with all-trans-retinoic acid and a synthetic retinoid., Kleywegt GJ, Bergfors T, Senn H, Le Motte P, Gsell B, Shudo K, Jones TA, Structure. 1994 Dec 15;2(12):1241-58. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7704533 7704533]
*[[Molecular Playground/CRABP I (Cellular Retinoic Acid Binding Protein)|Molecular Playground/CRABP I (Cellular Retinoic Acid Binding Protein)]]
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bergfors, T.]]
[[Category: Bergfors T]]
[[Category: Jones, T.A.]]
[[Category: Jones TA]]
[[Category: Kleywegt, G.J.]]
[[Category: Kleywegt GJ]]
[[Category: PO4]]
[[Category: RE9]]
[[Category: retinoic-acid transport]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:35:05 2008''

Latest revision as of 09:42, 7 February 2024

CRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOIDCRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOID

Structural highlights

1cbq is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RABP2_HUMAN Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1cbq, resolution 2.20Å

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