1c9s: Difference between revisions

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New page: left|200px<br /><applet load="1c9s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c9s, resolution 1.90Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1c9s.gif|left|200px]]<br /><applet load="1c9s" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1c9s, resolution 1.90&Aring;" />
'''CRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDES'''<br />


==Overview==
==CRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDES==
The trp RNA-binding attenuation protein (TRAP) regulates expression of the, tryptophan biosynthetic genes of several bacilli by binding, single-stranded RNA. The binding sequence is composed of eleven triplet, repeats, predominantly GAG, separated by two or three non-conserved, nucleotides. Here we present the crystal structure of a complex of TRAP, and a 53-base single-stranded RNA containing eleven GAG triplets, revealing that each triplet is accommodated in a binding pocket formed by, beta-strands. In the complex, the RNA has an extended structure without, any base-pairing and binds to the protein mostly by specific protein-base, interactions. Eleven binding pockets on the circular TRAP 11-mer form a, belt with a diameter of about 80 A. This simple but elegant mechanism of, arresting the RNA segment by encircling it around a protein disk is, applicable to both transcription, when TRAP binds the nascent RNA, and to, translation, when TRAP binds the same sequence within a non-coding leader, region of the messenger RNA.
<StructureSection load='1c9s' size='340' side='right'caption='[[1c9s]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1c9s]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C9S FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c9s OCA], [https://pdbe.org/1c9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c9s RCSB], [https://www.ebi.ac.uk/pdbsum/1c9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c9s ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MTRB_GEOSE MTRB_GEOSE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c9/1c9s_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c9s ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1C9S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with TRP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C9S OCA].
*[[Tryptophan RNA-binding attenuation protein|Tryptophan RNA-binding attenuation protein]]
 
__TOC__
==Reference==
</StructureSection>
Structure of the trp RNA-binding attenuation protein, TRAP, bound to RNA., Antson AA, Dodson EJ, Dodson G, Greaves RB, Chen X, Gollnick P, Nature. 1999 Sep 16;401(6750):235-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10499579 10499579]
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Antson, A.A.]]
[[Category: Antson AA]]
[[Category: Chen, X.P.]]
[[Category: Chen X-P]]
[[Category: Dodson, E.J.]]
[[Category: Dodson EJ]]
[[Category: Dodson, G.G.]]
[[Category: Dodson GG]]
[[Category: Gollnick, P.]]
[[Category: Gollnick P]]
[[Category: Greaves, R.B.]]
[[Category: Greaves RB]]
[[Category: TRP]]
[[Category: protein-rna complex]]
[[Category: single stranded rna]]
[[Category: transcription]]
[[Category: trap]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:17:33 2007''

Latest revision as of 09:41, 7 February 2024

CRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDESCRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDES

Structural highlights

1c9s is a 23 chain structure with sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.9Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MTRB_GEOSE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1c9s, resolution 1.90Å

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