1c9s: Difference between revisions

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-[[Image:1c9s.gif|left|200px]]
- {{Structure
+==CRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDES==
-|PDB= 1c9s |SIZE=350|CAPTION= <scene name='initialview01'>1c9s</scene>, resolution 1.90&Aring;
+<StructureSection load='1c9s' size='340' side='right'caption='[[1c9s]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene>
+<table><tr><td colspan='2'>[[1c9s]] is a 23 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C9S FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c9s OCA], [https://pdbe.org/1c9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c9s RCSB], [https://www.ebi.ac.uk/pdbsum/1c9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c9s ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/MTRB_GEOSE MTRB_GEOSE]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c9/1c9s_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c9s ConSurf].
+<div style="clear:both"></div>
-'''CRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDES'''
+==See Also==
+*[[Tryptophan RNA-binding attenuation protein|Tryptophan RNA-binding attenuation protein]]
+__TOC__
-==Overview==
+</StructureSection>
-The trp RNA-binding attenuation protein (TRAP) regulates expression of the tryptophan biosynthetic genes of several bacilli by binding single-stranded RNA. The binding sequence is composed of eleven triplet repeats, predominantly GAG, separated by two or three non-conserved nucleotides. Here we present the crystal structure of a complex of TRAP and a 53-base single-stranded RNA containing eleven GAG triplets, revealing that each triplet is accommodated in a binding pocket formed by beta-strands. In the complex, the RNA has an extended structure without any base-pairing and binds to the protein mostly by specific protein-base interactions. Eleven binding pockets on the circular TRAP 11-mer form a belt with a diameter of about 80 A. This simple but elegant mechanism of arresting the RNA segment by encircling it around a protein disk is applicable to both transcription, when TRAP binds the nascent RNA, and to translation, when TRAP binds the same sequence within a non-coding leader region of the messenger RNA.
-==About this Structure==
-C9S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C9S OCA].
-==Reference==
-Structure of the trp RNA-binding attenuation protein, TRAP, bound to RNA., Antson AA, Dodson EJ, Dodson G, Greaves RB, Chen X, Gollnick P, Nature. 1999 Sep 16;401(6750):235-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10499579 10499579]
 [[Category: Geobacillus stearothermophilus]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Antson, A A.]]
+[[Category: Antson AA]]
-[[Category: Chen, X P.]]
+[[Category: Chen X-P]]
-[[Category: Dodson, E J.]]
+[[Category: Dodson EJ]]
-[[Category: Dodson, G G.]]
+[[Category: Dodson GG]]
-[[Category: Gollnick, P.]]
+[[Category: Gollnick P]]
-[[Category: Greaves, R B.]]
+[[Category: Greaves RB]]
-[[Category: TRP]]
-[[Category: protein-rna complex]]
-[[Category: single stranded rna]]
-[[Category: transcription]]
-[[Category: trap]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:21:40 2008''