1c3m: Difference between revisions

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New page: left|200px<br /><applet load="1c3m" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c3m, resolution 2.0Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1c3m.gif|left|200px]]<br /><applet load="1c3m" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1c3m, resolution 2.0&Aring;" />
'''CRYSTAL STRUCTURE OF HELTUBA COMPLEXED TO MAN(1-3)MAN'''<br />


==Overview==
==CRYSTAL STRUCTURE OF HELTUBA COMPLEXED TO MAN(1-3)MAN==
BACKGROUND: Heltuba, a tuber lectin from the Jerusalem artichoke, Helianthus tuberosus, belongs to the mannose-binding subgroup of the, family of jacalin-related plant lectins. Heltuba is highly specific for, the disaccharides Man alpha 1-3Man or Man alpha 1-2Man, two carbohydrates, that are particularly abundant in the glycoconjugates exposed on the, surface of viruses, bacteria and fungi, and on the epithelial cells along, the gastrointestinal tract of lower animals. Heltuba is therefore a good, candidate as a defense protein against plant pathogens or predators., RESULTS: The 2.0 A resolution structure of Heltuba exhibits a threefold, symmetric beta-prism fold made up of three four-stranded beta sheets. The, crystal structures of Heltuba in complex with Man alpha 1-3Man and Man, alpha 1-2Man, solved at 2.35 A and 2.45 A resolution respectively, reveal, the carbohydrate-binding site and the residues required for the, specificity towards alpha 1-3 or alpha 1-2 mannose linkages. In addition, the crystal packing reveals a remarkable, donut-shaped, octahedral, assembly of subunits with the mannose moieties at the periphery, suggesting possible cross-linking interactions with branched, oligomannosides. CONCLUSIONS: The structure of Heltuba, which is the, prototype for an extended family of mannose-binding agglutinins, shares, the carbohydrate-binding site and beta-prism topology of its, galactose-binding counterparts jacalin and Maclura pomifera lectin., However, the beta-prism elements recruited to form the octameric interface, of Heltuba, and the strategy used to forge the mannose-binding site, are, unique and markedly dissimilar to those described for jacalin. The present, structure highlights a hitherto unrecognized adaptability of the, beta-prism building block in the evolution of plant proteins.
<StructureSection load='1c3m' size='340' side='right'caption='[[1c3m]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1c3m]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helianthus_tuberosus Helianthus tuberosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C3M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C3M FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=PRD_900112:3alpha-alpha-mannobiose'>PRD_900112</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c3m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c3m OCA], [https://pdbe.org/1c3m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c3m RCSB], [https://www.ebi.ac.uk/pdbsum/1c3m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c3m ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9ZQY5_HELTU Q9ZQY5_HELTU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c3/1c3m_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c3m ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1C3M is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Helianthus_tuberosus Helianthus tuberosus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C3M OCA].
*[[Agglutinin 3D structures|Agglutinin 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Helianthus tuberosus lectin reveals a widespread scaffold for mannose-binding lectins., Bourne Y, Zamboni V, Barre A, Peumans WJ, Van Damme EJ, Rouge P, Structure. 1999 Dec 15;7(12):1473-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10647178 10647178]
[[Category: Helianthus tuberosus]]
[[Category: Helianthus tuberosus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Barre, A.]]
[[Category: Barre A]]
[[Category: Bourne, Y.]]
[[Category: Bourne Y]]
[[Category: Damme, E.J.M.van.]]
[[Category: Peumans WJ]]
[[Category: Peumans, W.J.]]
[[Category: Rouge P]]
[[Category: Rouge, P.]]
[[Category: Zamboni V]]
[[Category: Zamboni, V.]]
[[Category: Van Damme EJM]]
[[Category: agglutinin]]
[[Category: beta-prism]]
[[Category: jacalin-related]]
[[Category: mannose]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 01:01:53 2007''

Latest revision as of 09:40, 7 February 2024

CRYSTAL STRUCTURE OF HELTUBA COMPLEXED TO MAN(1-3)MANCRYSTAL STRUCTURE OF HELTUBA COMPLEXED TO MAN(1-3)MAN

Structural highlights

1c3m is a 1 chain structure with sequence from Helianthus tuberosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9ZQY5_HELTU

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1c3m, resolution 2.00Å

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