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[[Image:1bsl.gif|left|200px]]


{{Structure
==STRUCTURE OF ALKANAL MONOOXYGENASE BETA CHAIN==
|PDB= 1bsl |SIZE=350|CAPTION= <scene name='initialview01'>1bsl</scene>, resolution 1.95&Aring;
<StructureSection load='1bsl' size='340' side='right'caption='[[1bsl]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1bsl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BSL FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bsl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bsl OCA], [https://pdbe.org/1bsl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bsl RCSB], [https://www.ebi.ac.uk/pdbsum/1bsl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bsl ProSAT]</span></td></tr>
}}
</table>
== Function ==
[https://www.uniprot.org/uniprot/LUXB_VIBHA LUXB_VIBHA] Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/1bsl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bsl ConSurf].
<div style="clear:both"></div>


'''STRUCTURE OF ALKANAL MONOOXYGENASE BETA CHAIN'''
==See Also==
 
*[[Luciferase 3D structures|Luciferase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Luciferase, as isolated from Vibrio harveyi, is an alpha beta heterodimer. When allowed to fold in the absence of the alpha subunit, either in vitro or in vivo, the beta subunit of enzyme will form a kinetically stable homodimer that does not unfold even after prolonged incubation in 5 M urea at pH 7.0 and 18 degrees C. This form of the beta subunit, arising via kinetic partitioning on the folding pathway, appears to constitute a kinetically trapped alternative to the heterodimeric enzyme (Sinclair JF, Ziegler MM, Baldwin TO. 1994. Kinetic partitioning during protein folding yields multiple native states. Nature Struct Biol 1: 320-326). Here we describe the X-ray crystal structure of the beta 2 homodimer of luciferase from V. harveyi determined and refined at 1.95 A resolution. Crystals employed in the investigational belonged to the orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions of a = 58.8 A, b = 62.0 A, and c = 218.2 A and contained one dimer per asymmetric unit. Like that observed in the functional luciferase alpha beta heterodimer, the major tertiary structural motif of each beta subunit consists of an (alpha/beta)8 barrel (Fisher AJ, Raushel FM, Baldwin TO, Rayment I. 1995. Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution. Biochemistry 34: 6581-6586). The root-mean-square deviation of the alpha-carbon coordinates between the beta subunits of the hetero- and homodimers is 0.7 A. This high resolution X-ray analysis demonstrated that "domain" or "loop" swapping has not occurred upon formation of the beta 2 homodimer and thus the stability of the beta 2 species to denaturation cannot be explained in such simple terms. In fact, the subunit:subunit interfaces observed in both the beta 2 homodimer and alpha beta heterodimer are remarkably similar in hydrogen-bonding patterns and buried surface areas.
[[Category: Large Structures]]
 
==About this Structure==
1BSL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_harveyi Vibrio harveyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BSL OCA].
 
==Reference==
Structure of the beta 2 homodimer of bacterial luciferase from Vibrio harveyi: X-ray analysis of a kinetic protein folding trap., Thoden JB, Holden HM, Fisher AJ, Sinclair JF, Wesenberg G, Baldwin TO, Rayment I, Protein Sci. 1997 Jan;6(1):13-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9007973 9007973]
[[Category: Alkanal monooxygenase (FMN-linked)]]
[[Category: Single protein]]
[[Category: Vibrio harveyi]]
[[Category: Vibrio harveyi]]
[[Category: Baldwin, T O.]]
[[Category: Baldwin TO]]
[[Category: Holden, H M.]]
[[Category: Holden HM]]
[[Category: Rayment, I.]]
[[Category: Rayment I]]
[[Category: Thoden, J B.]]
[[Category: Thoden JB]]
[[Category: flavoprotein]]
[[Category: fmn]]
[[Category: luminescence]]
[[Category: monooxygenase]]
[[Category: oxidoreductase]]
[[Category: photoprotein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:15:14 2008''

Latest revision as of 09:38, 7 February 2024

STRUCTURE OF ALKANAL MONOOXYGENASE BETA CHAINSTRUCTURE OF ALKANAL MONOOXYGENASE BETA CHAIN

Structural highlights

1bsl is a 2 chain structure with sequence from Vibrio harveyi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.95Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LUXB_VIBHA Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1bsl, resolution 1.95Å

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