1bou: Difference between revisions

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-[[Image:1bou.gif|left|200px]]<br />
-<applet load="1bou" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1bou, resolution 2.2&Aring;" />
-'''THREE-DIMENSIONAL STRUCTURE OF LIGAB'''<br />
-==Overview==
+==THREE-DIMENSIONAL STRUCTURE OF LIGAB==
-BACKGROUND: Sphingomonas paucimobilis SYK-6 utilizes an extradiol-type, catecholic dioxygenase, the LigAB enzyme (a protocatechuate, 4,5-dioxygenase), to oxidize protocatechuate (or 3,4-dihydroxybenzoic, acid, PCA). The enzyme belongs to the family of class III extradiol-type, catecholic dioxygenases catalyzing the ring-opening reaction of, protocatechuate and related compounds. The primary structure of LigAB, suggests that the enzyme has no evolutionary relationship with the family, of class II extradiol-type catecholic dioxygenases. Both the class II and, class III enzymes utilize a non-heme ferrous center for adding dioxygen to, the substrate. By elucidating the structure of LigAB, we aimed to provide, a structural basis for discussing the function of class III enzymes., RESULTS: The ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10467151 (full description)]]
+<StructureSection load='1bou' size='340' side='right'caption='[[1bou]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1bou]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOU FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bou OCA], [https://pdbe.org/1bou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bou RCSB], [https://www.ebi.ac.uk/pdbsum/1bou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bou ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PCYA_SPHSK PCYA_SPHSK]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bou_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bou ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-BOU is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Sphingomonas_paucimobilis Sphingomonas paucimobilis]] with FE as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Protocatechuate_4,5-dioxygenase Protocatechuate 4,5-dioxygenase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.8 1.13.11.8]]. Structure known Active Sites: FEA and FEB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BOU OCA]].
+*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structure of an aromatic ring opening dioxygenase LigAB, a protocatechuate 4,5-dioxygenase, under aerobic conditions., Sugimoto K, Senda T, Aoshima H, Masai E, Fukuda M, Mitsui Y, Structure. 1999 Aug 15;7(8):953-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10467151 10467151]
+[[Category: Large Structures]]
-[[Category: Protein complex]]
-[[Category: Protocatechuate 4,5-dioxygenase]]
 [[Category: Sphingomonas paucimobilis]]
-[[Category: Fukuda, M.]]
+[[Category: Fukuda M]]
-[[Category: Mitsui, Y.]]
+[[Category: Mitsui Y]]
-[[Category: Senda, T.]]
+[[Category: Senda T]]
-[[Category: Sugimoto, K.]]
+[[Category: Sugimoto K]]
-[[Category: FE]]
-[[Category: extradiol type dioxygenase]]
-[[Category: oxidoreductase]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:56:25 2007''