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| [[Image:1bi2.gif|left|200px]]
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| {{Structure
| | ==STRUCTURE OF APO-AND HOLO-DIPHTHERIA TOXIN REPRESSOR== |
| |PDB= 1bi2 |SIZE=350|CAPTION= <scene name='initialview01'>1bi2</scene>, resolution 2.3Å
| | <StructureSection load='1bi2' size='340' side='right'caption='[[1bi2]], [[Resolution|resolution]] 2.30Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[1bi2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BI2 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| |GENE= DTXR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1717 Corynebacterium diphtheriae])
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bi2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bi2 OCA], [https://pdbe.org/1bi2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bi2 RCSB], [https://www.ebi.ac.uk/pdbsum/1bi2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bi2 ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | == Function == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bi2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bi2 OCA], [http://www.ebi.ac.uk/pdbsum/1bi2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bi2 RCSB]</span>
| | [https://www.uniprot.org/uniprot/DTXR_CORDI DTXR_CORDI] Iron-binding repressor of the dipheteria toxin gene expression. May serve as a global regulator of gene expression. Represses ripA under iron excess. |
| }}
| | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] |
| | Check<jmol> |
| | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bi/1bi2_consurf.spt"</scriptWhenChecked> |
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> |
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bi2 ConSurf]. |
| | <div style="clear:both"></div> |
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| '''STRUCTURE OF APO-AND HOLO-DIPHTHERIA TOXIN REPRESSOR'''
| | ==See Also== |
| | | *[[Diphtheria toxin repressor|Diphtheria toxin repressor]] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| The diphtheria toxin repressor (DtxR) from Corynebacterium diphtheriae is a divalent metal-activated repressor of chromosomal genes that encode proteins responsible for siderophore-mediated iron uptake and also of the gene of certain corynebacteriophages that encodes diphtheria toxin. DtxR consists of two 25.3-kDa three-domain subunits and is a member of a family of related repressor proteins in several Gram-positive bacterial species, some of which are important human pathogens. In this paper, we report on the first high resolution crystal structures of apo-DtxR in two related space groups. In addition, crystal structures of Zn-DtxR were determined in the same two space groups. The resolutions of the structures range from 2.2 to 2.4 A. The four refined models of the apo- and the holo-repressor exhibit quite similar metal binding centers, which do, however, show higher thermal motion in the apo-structures. All four structures reported differ from each other in one important aspect. The N-terminal DNA-binding domain and the last 20 residues of the dimerization domain of each subunit move significantly with respect to the core of the DtxR dimer, which consists of residues 74-120 from both subunits. These results provide the first indication of a conformational change that may occur upon binding of the holo-repressor to DNA.
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| ==About this Structure==
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| 1BI2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BI2 OCA].
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| ==Reference==
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| Motion of the DNA-binding domain with respect to the core of the diphtheria toxin repressor (DtxR) revealed in the crystal structures of apo- and holo-DtxR., Pohl E, Holmes RK, Hol WG, J Biol Chem. 1998 Aug 28;273(35):22420-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9712865 9712865]
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| [[Category: Corynebacterium diphtheriae]] | | [[Category: Corynebacterium diphtheriae]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Hol, W G.J.]] | | [[Category: Hol WGJ]] |
| [[Category: Pohl, E.]] | | [[Category: Pohl E]] |
| [[Category: dna-binding]]
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| [[Category: iron]]
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| [[Category: repressor]]
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| [[Category: transcription regulation]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:01:21 2008''
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