1bhn: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1bhn.gif|left|200px]]


{{Structure
==NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA==
|PDB= 1bhn |SIZE=350|CAPTION= <scene name='initialview01'>1bhn</scene>, resolution 2.4&Aring;
<StructureSection load='1bhn' size='340' side='right'caption='[[1bhn]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
|SITE= <scene name='pdbsite=A:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>A</scene>, <scene name='pdbsite=AND F:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>AND F</scene>, <scene name='pdbsite=B:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>B</scene>, <scene name='pdbsite=C:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>C</scene>, <scene name='pdbsite=D:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>D</scene> and <scene name='pdbsite=E:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>E</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=35G:GUANOSINE-3&#39;,5&#39;-MONOPHOSPHATE'>35G</scene>, <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>
<table><tr><td colspan='2'>[[1bhn]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BHN FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=35G:GUANOSINE-3,5-MONOPHOSPHATE'>35G</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bhn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bhn OCA], [https://pdbe.org/1bhn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bhn RCSB], [https://www.ebi.ac.uk/pdbsum/1bhn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bhn ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bhn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bhn OCA], [http://www.ebi.ac.uk/pdbsum/1bhn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bhn RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/NDKA1_BOVIN NDKA1_BOVIN]
 
== Evolutionary Conservation ==
'''NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/1bhn_consurf.spt"</scriptWhenChecked>
The crystal structures of two isoforms of nucleoside diphosphate kinase from bovine retina overexpressed in Escherischia coli have been determined to 2.4 A resolution. Both the isoforms, NBR-A and NBR-B, are hexameric and the fold of the monomer is in agreement with NDP-kinase structures from other biological sources. Although the polypeptide chains of the two isoforms differ by only two residues, they crystallize in different space groups. NBR-A crystallizes in space group P212121 with an entire hexamer in the asymmetric unit, while NBR-B crystallizes in space group P43212 with a trimer in the asymmetric unit. The highly conserved nucleotide-binding site observed in other nucleoside diphosphate kinase structures is also observed here. Both NBR-A and NBR-B were crystallized in the presence of cGMP. The nucleotide is bound with the base in the anti conformation. The NBR-A active site contained both cGMP and GDP each bound at half occupancy. Presumably, NBR-A had retained GDP (or GTP) from the purification process. The NBR-B active site contained only cGMP.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1BHN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BHN OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bhn ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
The three-dimensional structures of two isoforms of nucleoside diphosphate kinase from bovine retina., Ladner JE, Abdulaev NG, Kakuev DL, Tordova M, Ridge KD, Gilliland GL, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1127-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10329774 10329774]
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Nucleoside-diphosphate kinase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Abdulaev NG]]
[[Category: Abdulaev, N G.]]
[[Category: Eisenstein E]]
[[Category: Eisenstein, E.]]
[[Category: Fujiwara JH]]
[[Category: Fujiwara, J H.]]
[[Category: Gilliland GL]]
[[Category: Gilliland, G L.]]
[[Category: Kakuev DL]]
[[Category: Kakuev, D L.]]
[[Category: Karaschuk GN]]
[[Category: Karaschuk, G N.]]
[[Category: Ladner JE]]
[[Category: Ladner, J E.]]
[[Category: Ridge KD]]
[[Category: Ridge, K D.]]
[[Category: Tordova M]]
[[Category: Tordova, M.]]
[[Category: phosphotransferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:01:22 2008''

Latest revision as of 09:36, 7 February 2024

NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINANUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA

Structural highlights

1bhn is a 6 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.4Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NDKA1_BOVIN

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1bhn, resolution 2.40Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1bhn&oldid=4040603"