|
|
| (13 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| [[Image:1baz.gif|left|200px]]
| |
|
| |
|
| {{Structure
| | ==ARC REPRESSOR MUTANT PHE10VAL== |
| |PDB= 1baz |SIZE=350|CAPTION= <scene name='initialview01'>1baz</scene>, resolution 1.9Å
| | <StructureSection load='1baz' size='340' side='right'caption='[[1baz]], [[Resolution|resolution]] 1.90Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[1baz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BAZ FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| |GENE= ARC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10754 Enterobacteria phage P22])
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1baz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1baz OCA], [https://pdbe.org/1baz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1baz RCSB], [https://www.ebi.ac.uk/pdbsum/1baz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1baz ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | == Function == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1baz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1baz OCA], [http://www.ebi.ac.uk/pdbsum/1baz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1baz RCSB]</span>
| | [https://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22] This protein acts as a transcriptional repressor of its own gene arc and of gene ant. |
| }}
| | __TOC__ |
| | | </StructureSection> |
| '''ARC REPRESSOR MUTANT PHE10VAL'''
| | [[Category: Large Structures]] |
| | | [[Category: Salmonella virus P22]] |
| | | [[Category: Raumann BE]] |
| ==Overview==
| | [[Category: Sauer RT]] |
| A central question in protein-DNA recognition is the origin of the specificity that permits binding to the correct site in the presence of excess, nonspecific DNA. In the P22 Arc repressor, the Phe-10 side chain is part of the hydrophobic core of the free protein but rotates out to pack against the sugar-phosphate backbone of the DNA in the repressor-operator complex. Characterization of a library of position 10 variants reveals that Phe is the only residue that results in fully active Arc. One class of mutants folds stably but binds operator with reduced affinity; another class is unstable. FV10, one member of the first class, binds operator DNA and nonoperator DNA almost equally well. The affinity differences between FV10 and wild type indicate that each Phe-10 side chain contributes 1.5-2.0 kcal to operator binding but less than 0.5 kcal/mol to nonoperator binding, demonstrating that contacts between Phe-10 and the operator DNA backbone contribute to binding specificity. This appears to be a direct contribution as the crystal structure of the FV10 dimer is similar to wild type and the Phe-10-DNA backbone interactions are the only contacts perturbed in the cocrystal structure of the FV10-operator complex.
| | [[Category: Schildbach JF]] |
| | |
| ==About this Structure==
| |
| 1BAZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BAZ OCA].
| |
| | |
| ==Reference== | |
| Origins of DNA-binding specificity: role of protein contacts with the DNA backbone., Schildbach JF, Karzai AW, Raumann BE, Sauer RT, Proc Natl Acad Sci U S A. 1999 Feb 2;96(3):811-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9927650 9927650]
| |
| [[Category: Enterobacteria phage p22]] | |
| [[Category: Single protein]] | |
| [[Category: Raumann, B E.]] | |
| [[Category: Sauer, R T.]] | |
| [[Category: Schildbach, J F.]] | |
| [[Category: transcription regulation]]
| |
| | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:57:22 2008''
| |