1azp: Difference between revisions

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[[Image:1azp.gif|left|200px]]


{{Structure
==HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SAC7D BOUND WITH KINKED DNA DUPLEX==
|PDB= 1azp |SIZE=350|CAPTION= <scene name='initialview01'>1azp</scene>, resolution 1.6&Aring;
<StructureSection load='1azp' size='340' side='right'caption='[[1azp]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND=
<table><tr><td colspan='2'>[[1azp]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AZP FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
|GENE=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1azp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1azp OCA], [https://pdbe.org/1azp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1azp RCSB], [https://www.ebi.ac.uk/pdbsum/1azp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1azp ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SAC7D BOUND WITH KINKED DNA DUPLEX'''
[https://www.uniprot.org/uniprot/DN7D_SULAC DN7D_SULAC] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990]
 
__TOC__
 
</StructureSection>
==Overview==
[[Category: Large Structures]]
The proteins Sac7d and Sso7d belong to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius and S. solfactaricus, respectively. These proteins are extremely stable to heat, acid and chemical agents. Sac7d binds to DNA without any particular sequence preference and thereby increases its melting temperature by approximately 40 degrees C. We have now solved and refined the crystal structure of Sac7d in complex with two DNA sequences to high resolution. The structures are examples of a nonspecific DNA-binding protein bound to DNA, and reveal that Sac7d binds in the minor groove, causing a sharp kinking of the DNA helix that is more marked than that induced by any sequence-specific DNA-binding proteins. The kink results from the intercalation of specific hydrophobic side chains of Sac7d into the DNA structure, but without causing any significant distortion of the protein structure relative to the uncomplexed protein in solution.
 
==About this Structure==
1AZP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZP OCA].  
 
==Reference==
The hyperthermophile chromosomal protein Sac7d sharply kinks DNA., Robinson H, Gao YG, McCrary BS, Edmondson SP, Shriver JW, Wang AH, Nature. 1998 Mar 12;392(6672):202-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9515968 9515968]
[[Category: Single protein]]
[[Category: Sulfolobus acidocaldarius]]
[[Category: Sulfolobus acidocaldarius]]
[[Category: Edmondson, S P.]]
[[Category: Edmondson SP]]
[[Category: Gao, Y G.]]
[[Category: Gao Y-G]]
[[Category: Mccrary, B S.]]
[[Category: Mccrary BS]]
[[Category: Robinson, H.]]
[[Category: Robinson H]]
[[Category: Shriver, J W.]]
[[Category: Shriver JW]]
[[Category: Wang, A H.J.]]
[[Category: Wang AH-J]]
[[Category: archea kinked-dna]]
[[Category: complex (chromatin protein/dna)]]
[[Category: dna-binding]]
[[Category: intercalation]]
[[Category: minor-groove binding]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:04:21 2008''

Latest revision as of 09:34, 7 February 2024

HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SAC7D BOUND WITH KINKED DNA DUPLEXHYPERTHERMOPHILE CHROMOSOMAL PROTEIN SAC7D BOUND WITH KINKED DNA DUPLEX

Structural highlights

1azp is a 3 chain structure with sequence from Sulfolobus acidocaldarius. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DN7D_SULAC Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990]

1azp, resolution 1.60Å

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OCA
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