1awi: Difference between revisions

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-[[Image:1awi.gif|left|200px]]<br /><applet load="1awi" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1awi, resolution 2.2&Aring;" />
-'''HUMAN PLATELET PROFILIN COMPLEXED WITH THE L-PRO10 PEPTIDE'''<br />
-==Overview==
+==HUMAN PLATELET PROFILIN COMPLEXED WITH THE L-PRO10 PEPTIDE==
-Profilin, a ubiquitous low molecular weight (13,000-15,000 M(r)) actin, binding protein, regulates the formation of F-actin structures in vivo, and is localized to specific cellular regions through interaction with, proline-rich sequences. Here we report the 2.2 A X-ray structure of the, complex between human platelet profilin (HPP) and a decamer of L-proline, (L-Pro10). The L-Pro10 peptide adopts a left-handed type II poly-L-proline, helix (PPII) and binds to a highly conserved patch of aromatic amino acids, on the surface of profilin. The peptide and actin binding sites reside on, orthogonal surfaces, and L-Pro10 binding does not result in a, conformational rearrangement of HPP. This structure suggests a mechanism, for the localization of profilin and its actin-related activities to sites, of actin filament assembly in vivo.
+<StructureSection load='1awi' size='340' side='right'caption='[[1awi]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1awi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AWI FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1awi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1awi OCA], [https://pdbe.org/1awi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1awi RCSB], [https://www.ebi.ac.uk/pdbsum/1awi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1awi ProSAT]</span></td></tr>
+</table>
+== Disease ==
+[https://www.uniprot.org/uniprot/PROF1_HUMAN PROF1_HUMAN] Defects in PFN1 are the cause of amyotrophic lateral sclerosis 18 (ALS18) [MIM:[https://omim.org/entry/614808 614808]. A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.<ref>PMID:22801503</ref>
+== Function ==
+[https://www.uniprot.org/uniprot/PROF1_HUMAN PROF1_HUMAN] Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.<ref>PMID:18573880</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aw/1awi_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1awi ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-AWI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Known structural/functional Sites: <scene name='pdbsite=PPA:Poly-L-PRO+Binding+Site'>PPA</scene> and <scene name='pdbsite=PPB:Poly-L-PRO+Binding+Site'>PPB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AWI OCA].
+*[[Profilin 3D Structures|Profilin 3D Structures]]
+== References ==
-==Reference==
+<references/>
-Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation., Mahoney NM, Janmey PA, Almo SC, Nat Struct Biol. 1997 Nov;4(11):953-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9360613 9360613]
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Almo, S.C.]]
+[[Category: Almo SC]]
-[[Category: Mahoney, N.M.]]
+[[Category: Mahoney NM]]
-[[Category: actin cytoskeleton]]
-[[Category: complex (actin-binding protein/peptide)]]
-[[Category: poly-l-proline]]
-[[Category: profilin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb  3 09:31:54 2008''