1avq: Difference between revisions

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-[[Image:1avq.gif|left|200px]]<br /><applet load="1avq" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1avq, resolution 2.4&Aring;" />
-'''TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS'''<br />
-==Overview==
+==TOROIDAL STRUCTURE OF LAMBDA EXONUCLEASE DETERMINED AT 2.4 ANGSTROMS==
-Structure determination at 2.4 angstrom resolution shows that lambda-exonuclease consists of three subunits that form a toroid. The central channel is funnel shaped, tapering from an inner diameter of about 30 angstroms at the wider end to 15 angstroms at the narrow end. This is adequate to accommodate the DNA substrate and thus provides a structural basis for the ability of the enzyme to sequentially hydrolyze thousands of nucleotides in a highly processive manner. The results also suggest the locations of the active sites and the constraints that limit cleavage to a single strand.
+<StructureSection load='1avq' size='340' side='right'caption='[[1avq]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1avq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AVQ FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1avq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1avq OCA], [https://pdbe.org/1avq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1avq RCSB], [https://www.ebi.ac.uk/pdbsum/1avq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1avq ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/EXO_LAMBD EXO_LAMBD] Facilitates phage DNA recombination through the double-strand break repair (DSBR) and single-strand annealing pathways. Also important for the late, rolling-circle mode of lambda DNA replication.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/av/1avq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1avq ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-AVQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=ACT:'>ACT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Exodeoxyribonuclease_(lambda-induced) Exodeoxyribonuclease (lambda-induced)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.3 3.1.11.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AVQ OCA].
+*[[Exonuclease 3D structures|Exonuclease 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Toroidal structure of lambda-exonuclease., Kovall R, Matthews BW, Science. 1997 Sep 19;277(5333):1824-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9295273 9295273]
+[[Category: Escherichia virus Lambda]]
-[[Category: Enterobacteria phage lambda]]
+[[Category: Large Structures]]
-[[Category: Exodeoxyribonuclease (lambda-induced)]]
+[[Category: Kovall RA]]
-[[Category: Single protein]]
+[[Category: Matthews BW]]
-[[Category: Kovall, R A.]]
-[[Category: Matthews, B W.]]
-[[Category: ACT]]
-[[Category: PO4]]
-[[Category: 5'-3' exonuclease]]
-[[Category: deoxyribonuclease]]
-[[Category: dna recombination and repair]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:48:46 2008''