1akl: Difference between revisions

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-[[Image:1akl.gif|left|200px]]
- {{Structure
+==ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080==
-|PDB= 1akl |SIZE=350|CAPTION= <scene name='initialview01'>1akl</scene>, resolution 2.0&Aring;
+<StructureSection load='1akl' size='340' side='right'caption='[[1akl]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
+<table><tr><td colspan='2'>[[1akl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKL FirstGlance]. <br>
-|ACTIVITY= [http://en.wikipedia.org/wiki/Serralysin Serralysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.40 3.4.24.40]
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-}}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akl OCA], [https://pdbe.org/1akl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akl RCSB], [https://www.ebi.ac.uk/pdbsum/1akl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akl ProSAT]</span></td></tr>
+</table>
-'''ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080'''
+== Function ==
+[https://www.uniprot.org/uniprot/APRA_PSEAE APRA_PSEAE]
+== Evolutionary Conservation ==
-==Overview==
+[[Image:Consurf_key_small.gif|200px|right]]
-The crystal structure of the unliganded alkaline protease from Pseudomonas aeruginosa IFO3080 has been determined at 2.0 A resolution by the X-ray method. The enzyme consists of N-terminal catalytic and C-terminal beta-helix domains. On structural comparison between the present unliganded enzyme and structurally- known liganded enzyme, some structural changes were observed around the active site. In the unliganded enzyme, Y216 serves as the fifth ligand for the active site zinc ion. On ligand binding, Y216 may move to form a hydrogen-bond with the carbonyl oxygen of the P1 residue of a ligand peptide. D191 in the flexible loop, Y190 to D196, over the active site cleft forms hydrogen-bonds with the backbone atoms of the P1 and P2 residues of the ligand to close the entrance to the cleft. The water molecule which is the fourth ligand for the zinc ion is replaced by the carbonyl oxygen of the P1 residue. These structural changes around the active site may reflect the substrate-binding mode during the enzymatic reaction.
+Check<jmol>
+  <jmolCheckbox>
-==About this Structure==
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ak/1akl_consurf.spt"</scriptWhenChecked>
-AKL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKL OCA].
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
-==Reference==
+  </jmolCheckbox>
-Crystal structure of the unliganded alkaline protease from Pseudomonas aeruginosa IFO3080 and its conformational changes on ligand binding., Miyatake H, Hata Y, Fujii T, Hamada K, Morihara K, Katsube Y, J Biochem. 1995 Sep;118(3):474-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8690704 8690704]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1akl ConSurf].
+<div style="clear:both"></div>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Pseudomonas aeruginosa]]
-[[Category: Serralysin]]
+[[Category: Fujii T]]
-[[Category: Single protein]]
+[[Category: Hamada K]]
-[[Category: Fujii, T.]]
+[[Category: Hata Y]]
-[[Category: Hamada, K.]]
+[[Category: Katsube Y]]
-[[Category: Hata, Y.]]
+[[Category: Miyatake H]]
-[[Category: Katsube, Y.]]
+[[Category: Morihara K]]
-[[Category: Miyatake, H.]]
-[[Category: Morihara, K.]]
-[[Category: CA]]
-[[Category: ZN]]
-[[Category: hydrolase (metalloproteinase)]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:58:50 2008''