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[[Image:1akl.gif|left|200px]]<br /><applet load="1akl" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1akl, resolution 2.0&Aring;" />
'''ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080'''<br />


==Overview==
==ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080==
The crystal structure of the unliganded alkaline protease from Pseudomonas aeruginosa IFO3080 has been determined at 2.0 A resolution by the X-ray method. The enzyme consists of N-terminal catalytic and C-terminal beta-helix domains. On structural comparison between the present unliganded enzyme and structurally- known liganded enzyme, some structural changes were observed around the active site. In the unliganded enzyme, Y216 serves as the fifth ligand for the active site zinc ion. On ligand binding, Y216 may move to form a hydrogen-bond with the carbonyl oxygen of the P1 residue of a ligand peptide. D191 in the flexible loop, Y190 to D196, over the active site cleft forms hydrogen-bonds with the backbone atoms of the P1 and P2 residues of the ligand to close the entrance to the cleft. The water molecule which is the fourth ligand for the zinc ion is replaced by the carbonyl oxygen of the P1 residue. These structural changes around the active site may reflect the substrate-binding mode during the enzymatic reaction.
<StructureSection load='1akl' size='340' side='right'caption='[[1akl]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1akl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AKL FirstGlance]. <br>
1AKL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Serralysin Serralysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.40 3.4.24.40] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AKL OCA].
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1akl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1akl OCA], [https://pdbe.org/1akl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1akl RCSB], [https://www.ebi.ac.uk/pdbsum/1akl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1akl ProSAT]</span></td></tr>
Crystal structure of the unliganded alkaline protease from Pseudomonas aeruginosa IFO3080 and its conformational changes on ligand binding., Miyatake H, Hata Y, Fujii T, Hamada K, Morihara K, Katsube Y, J Biochem. 1995 Sep;118(3):474-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8690704 8690704]
</table>
== Function ==
[https://www.uniprot.org/uniprot/APRA_PSEAE APRA_PSEAE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ak/1akl_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1akl ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Serralysin]]
[[Category: Fujii T]]
[[Category: Single protein]]
[[Category: Hamada K]]
[[Category: Fujii, T.]]
[[Category: Hata Y]]
[[Category: Hamada, K.]]
[[Category: Katsube Y]]
[[Category: Hata, Y.]]
[[Category: Miyatake H]]
[[Category: Katsube, Y.]]
[[Category: Morihara K]]
[[Category: Miyatake, H.]]
[[Category: Morihara, K.]]
[[Category: CA]]
[[Category: ZN]]
[[Category: hydrolase (metalloproteinase)]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:45:33 2008''

Latest revision as of 09:31, 7 February 2024

ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080

Structural highlights

1akl is a 1 chain structure with sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

APRA_PSEAE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1akl, resolution 2.00Å

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