1aei: Difference between revisions

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-[[Image:1aei.jpg|left|200px]]<br /><applet load="1aei" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="1aei, resolution 2.8&Aring;" />
-'''CRYSTAL STRUCTURE OF THE ANNEXIN XII HEXAMER'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE ANNEXIN XII HEXAMER==
-Annexins are a family of calcium- and phospholipid-binding proteins implicated in a number of biological processes including membrane fusion and ion channel formation. The crystal structure of the annexin XII hexamer, refined at 2.8 A resolution, forms a concave disk with 3-2 symmetry, about 100 A in diameter and 70 A thick with a central hydrophilic pore. Six intermolecular Ca2+ ions are involved in hexamer formation. An additional 18 Ca2+ ions are located on the perimeter of the disk, accessible only from the side of the hexameric disk. On the basis of the hexamer structure we propose here a new mode of protein-phospholipid bilayer interaction that is distinct from the hydrophobic insertion of typical membrane proteins. This speculative model postulates the Ca(2+)-dependent insertion of the hydrophilic annexin XII hexamer into phospholipid bilayers with local reorientation of the bilayer phospholipids.
+<StructureSection load='1aei' size='340' side='right'caption='[[1aei]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1aei]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Hydra_vulgaris Hydra vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AEI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AEI FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aei OCA], [https://pdbe.org/1aei PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aei RCSB], [https://www.ebi.ac.uk/pdbsum/1aei PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aei ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/ANX12_HYDVU ANX12_HYDVU]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ae/1aei_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aei ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-AEI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Hydra_vulgaris Hydra vulgaris] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AEI OCA].
+*[[Annexin 3D structures|Annexin 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structure of the annexin XII hexamer and implications for bilayer insertion., Luecke H, Chang BT, Mailliard WS, Schlaepfer DD, Haigler HT, Nature. 1995 Nov 30;378(6556):512-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7477411 7477411]
 [[Category: Hydra vulgaris]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Chang, B T.]]
+[[Category: Chang BT]]
-[[Category: Haigler, H T.]]
+[[Category: Haigler HT]]
-[[Category: Luecke, H.]]
+[[Category: Luecke H]]
-[[Category: Mailliard, W S.]]
+[[Category: Mailliard WS]]
-[[Category: Schlaepfer, D D.]]
+[[Category: Schlaepfer DD]]
-[[Category: CA]]
-[[Category: annexin]]
-[[Category: calcium]]
-[[Category: calcium/phospholipid-binding]]
-[[Category: phospholipid]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:43:37 2008''