1adv: Difference between revisions

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New page: left|200px<br /><applet load="1adv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1adv, resolution 3.2Å" /> '''EARLY E2A DNA-BINDING...
 
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[[Image:1adv.gif|left|200px]]<br /><applet load="1adv" size="450" color="white" frame="true" align="right" spinBox="true"
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'''EARLY E2A DNA-BINDING PROTEIN'''<br />


==Overview==
==EARLY E2A DNA-BINDING PROTEIN==
A second crystal form of the C-terminal domain of the adenovirus, single-stranded DNA binding protein crystallizes in space group, P2(1)2(1)2(1) with a=61.0 angstrom, b=91.2 angstrom and c=149.4 angstrom., The crystals contain two molecules per asymmetric unit and diffract to a, maximum resolution of 3.0 angstrom. The crystal is composed of infinite, chains of molecules along the crystallographic 2(1) axis parallel to c., The principal intermolecular interaction is a hooking of the C-terminal 17, residues of one molecule onto the next molecule in the protein chain., Adjacent molecules in the chain are rotated approximately 90 degrees with, respect to their neighbours. The difference in relative orientation of, adjacent molecules between the two crystal forms of the protein implies a, degree of flexibility in the protein chain that would facilitate DNA, binding.
<StructureSection load='1adv' size='340' side='right'caption='[[1adv]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1adv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_5 Human adenovirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ADV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ADV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1adv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1adv OCA], [https://pdbe.org/1adv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1adv RCSB], [https://www.ebi.ac.uk/pdbsum/1adv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1adv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DNB2_ADE05 DNB2_ADE05] Binds cooperatively single-stranded DNA in a sequence-independent manner. Involved in DNA-replication, regulation of mRNA formation, and host-range specificity. Zinc is required for DNA binding.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ad/1adv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1adv ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1ADV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_adenovirus_11 Human adenovirus 11] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ADV OCA].
*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Alternative arrangements of the protein chain are possible for the adenovirus single-stranded DNA binding protein., Kanellopoulos PN, Tsernoglou D, van der Vliet PC, Tucker PA, J Mol Biol. 1996 Mar 22;257(1):1-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8632448 8632448]
[[Category: Human adenovirus 5]]
[[Category: Human adenovirus 11]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Kanellopoulos PN]]
[[Category: Kanellopoulos, P.N.]]
[[Category: Tsernoglou D]]
[[Category: Tsernoglou, D.]]
[[Category: Tucker PA]]
[[Category: Tucker, P.A.]]
[[Category: Van Der Vliet PC]]
[[Category: Vliet, P.C.Van.Der.]]
[[Category: ZN]]
[[Category: dna-binding protein]]
[[Category: ssdna binding protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:47:02 2007''

Latest revision as of 09:29, 7 February 2024

EARLY E2A DNA-BINDING PROTEINEARLY E2A DNA-BINDING PROTEIN

Structural highlights

1adv is a 2 chain structure with sequence from Human adenovirus 5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DNB2_ADE05 Binds cooperatively single-stranded DNA in a sequence-independent manner. Involved in DNA-replication, regulation of mRNA formation, and host-range specificity. Zinc is required for DNA binding.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1adv, resolution 3.20Å

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