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[[Image:1a7j.gif|left|200px]]<br />
<applet load="1a7j" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1a7j, resolution 2.5&Aring;" />
'''PHOSPHORIBULOKINASE FROM RHODOBACTER SPHEROIDES'''<br />


==Overview==
==PHOSPHORIBULOKINASE FROM RHODOBACTER SPHEROIDES==
The essential photosynthetic enzyme phosphoribulokinase (PRK) is, responsible for the conversion of ribulose 5-phosphate (Ru5P) to ribulose, 1,5-bisphosphate, the substrate for the CO2 fixing enzyme ribulose, 1,5-bisphosphate carboxylase/oxygenase (Rubisco). We have determined the, structure of the octameric bacterial form of PRK to a resolution of 2.5 A., The protein is folded into a seven-member mixed beta-sheet surrounded by, alpha-helices, giving the overall appearance of the nucleotide, monophosphate family of kinases. Homology with the nucleotide, monophosphate kinases suggests a number of amino acid residues that are, likely to be important in catalysis and suggests the roles of some amino, acid residues that have been mutated prior to the determination of the, structure. Further, sequence identity across eukaryotic and prokaryotic, species and a calculation of the buried surface area suggests the identity, within the octamer of a dimer conserved throughout evolution. The width of, the groove leading to the active site is consistent with an oriented, molecule of thioredoxin controlling the oxidation state of two cysteines, that regulate activity in the eukaryotic enzymes. Although neither Asp 42, nor Asp 169 can be definitively assigned as the catalytic base, the, crystal structure suggests the location of a ribulose 5-phosphate binding, site and suggests a role for several of the conserved basic residues.
<StructureSection load='1a7j' size='340' side='right'caption='[[1a7j]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1a7j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A7J FirstGlance]. <br>
1A7J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phosphoribulokinase Phosphoribulokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.19 2.7.1.19] Structure known Active Site: CIC. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A7J OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a7j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a7j OCA], [https://pdbe.org/1a7j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a7j RCSB], [https://www.ebi.ac.uk/pdbsum/1a7j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a7j ProSAT]</span></td></tr>
The crystal structure of phosphoribulokinase from Rhodobacter sphaeroides reveals a fold similar to that of adenylate kinase., Harrison DH, Runquist JA, Holub A, Miziorko HM, Biochemistry. 1998 Apr 14;37(15):5074-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9548738 9548738]
</table>
[[Category: Phosphoribulokinase]]
== Function ==
[[Category: Rhodobacter sphaeroides]]
[https://www.uniprot.org/uniprot/KPPR1_CERSP KPPR1_CERSP]  
[[Category: Single protein]]
== Evolutionary Conservation ==
[[Category: Harrison, D.H.T.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Holub, A.]]
Check<jmol>
[[Category: Miziorko, H.]]
  <jmolCheckbox>
[[Category: Runquist, J.]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/1a7j_consurf.spt"</scriptWhenChecked>
[[Category: SO4]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: calvin cycle]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: kinase]]
  </jmolCheckbox>
[[Category: transferase]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a7j ConSurf].
 
<div style="clear:both"></div>
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 15:45:41 2007''
__TOC__
</StructureSection>
[[Category: Cereibacter sphaeroides]]
[[Category: Large Structures]]
[[Category: Harrison DHT]]
[[Category: Holub A]]
[[Category: Miziorko H]]
[[Category: Runquist J]]

Latest revision as of 09:28, 7 February 2024

PHOSPHORIBULOKINASE FROM RHODOBACTER SPHEROIDESPHOSPHORIBULOKINASE FROM RHODOBACTER SPHEROIDES

Structural highlights

1a7j is a 1 chain structure with sequence from Cereibacter sphaeroides. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.5Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KPPR1_CERSP

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1a7j, resolution 2.50Å

Drag the structure with the mouse to rotate

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OCA
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