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[[Image:1a7d.gif|left|200px]]<br /><applet load="1a7d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1a7d, resolution 1.8&Aring;" />
'''CHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLA'''<br />


==Overview==
==CHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLA==
Myohemerythrin (Mhr) is a nonheme iron oxygen carrier found in the retractor muscles of marine "peanut" worms. The X-ray crystal structures of two recombinant Themiste zostericola Mhrs are reported to a resolution of 1.8 A. Surprisingly, the met wild-type structure (R = 17.8%) was found to contain chloride bound to Fe2, while coordinated hydroxide was found in the met L103N structure (R = 18.3%). An internal water molecule was also found distal to the Fe-O-Fe center of the mutant protein, forming hydrogen bonds with the coordinated hydroxide and the OD1 atom of Asn-103. This finding is consistent with the kinetic and spectroscopic results reported for the L103N mutant Mhr [Raner, G. M., Martins, L. J., &amp; Ellis, W. R., Jr. (1997) Biochemistry 36, 7037-7043]. Possible roles for the side chain of residue 103 (Leu in wild-type Mhr) in gating ligand binding are also discussed.
<StructureSection load='1a7d' size='340' side='right'caption='[[1a7d]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1a7d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Themiste_hennahi Themiste hennahi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A7D FirstGlance]. <br>
1A7D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Themiste_zostericola Themiste zostericola] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=CFO:'>CFO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A7D OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CFO:CHLORO+DIIRON-OXO+MOIETY'>CFO</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a7d OCA], [https://pdbe.org/1a7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a7d RCSB], [https://www.ebi.ac.uk/pdbsum/1a7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a7d ProSAT]</span></td></tr>
Structures of wild-type chloromet and L103N hydroxomet Themiste zostericola myohemerythrins at 1.8 A resolution., Martins LJ, Hill CP, Ellis WR Jr, Biochemistry. 1997 Jun 10;36(23):7044-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9188702 9188702]
</table>
[[Category: Single protein]]
== Function ==
[[Category: Themiste zostericola]]
[https://www.uniprot.org/uniprot/HEMTM_THEHE HEMTM_THEHE] Myohemerythrin is an oxygen-binding protein found in the retractor muscles of certain worms. The oxygen-binding site contains two iron atoms.
[[Category: Hill, C P.]]
== Evolutionary Conservation ==
[[Category: Junior, W R.Ellis.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Martins, L J.]]
Check<jmol>
[[Category: CFO]]
  <jmolCheckbox>
[[Category: CL]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a7/1a7d_consurf.spt"</scriptWhenChecked>
[[Category: nonheme iron oxygen carrier]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: oxygen transport]]
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:41:42 2008''
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a7d ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Themiste hennahi]]
[[Category: Ellis Junior WR]]
[[Category: Hill CP]]
[[Category: Martins LJ]]

Latest revision as of 09:28, 7 February 2024

CHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLACHLOROMET MYOHEMERYTHRIN FROM THEMISTE ZOSTERICOLA

Structural highlights

1a7d is a 1 chain structure with sequence from Themiste hennahi. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HEMTM_THEHE Myohemerythrin is an oxygen-binding protein found in the retractor muscles of certain worms. The oxygen-binding site contains two iron atoms.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

1a7d, resolution 1.80Å

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