1a5t: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
New page: left|200px<br /> <applet load="1a5t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a5t, resolution 2.2Å" /> '''CRYSTAL STRUCTURE OF...
 
No edit summary
 
(22 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1a5t.gif|left|200px]]<br />
<applet load="1a5t" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1a5t, resolution 2.2&Aring;" />
'''CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III'''<br />


==Overview==
==CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III==
The crystal structure of the delta' subunit of the clamp-loader complex of, E. coli DNA polymerase III has been determined. Three consecutive domains, in the structure are arranged in a C-shaped architecture. The N-terminal, domain contains a nonfunctional nucleotide binding site. The catalytic, component of the clamp-loader complex is the gamma subunit, which is, homologous to delta'. A sequence-structure alignment suggests that, nucleotides bind to gamma at an interdomain interface within the inner, surface of the "C." The alignment is extended to other clamp-loader, complexes and to the RuvB family of DNA helicases, and suggests that each, of these is assembled from C-shaped components that can open and close the, jaws of the "C" in response to ATP binding and hydrolysis.
<StructureSection load='1a5t' size='340' side='right'caption='[[1a5t]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1a5t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A5T FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a5t OCA], [https://pdbe.org/1a5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a5t RCSB], [https://www.ebi.ac.uk/pdbsum/1a5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a5t ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HOLB_ECOLI HOLB_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/1a5t_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a5t ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1A5T is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with ZN as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A5T OCA]].
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of the delta' subunit of the clamp-loader complex of E. coli DNA polymerase III., Guenther B, Onrust R, Sali A, O'Donnell M, Kuriyan J, Cell. 1997 Oct 31;91(3):335-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9363942 9363942]
[[Category: Escherichia coli K-12]]
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Guenther B]]
[[Category: Donnell, M.O.]]
[[Category: Kuriyan J]]
[[Category: Guenther, B.]]
[[Category: O'Donnell M]]
[[Category: Kuriyan, J.]]
[[Category: Onrust R]]
[[Category: Onrust, R.]]
[[Category: Sali A]]
[[Category: Sali, A.]]
[[Category: ZN]]
[[Category: dna replication]]
[[Category: zinc finger]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:28:03 2007''

Latest revision as of 09:27, 7 February 2024

CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE IIICRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III

Structural highlights

1a5t is a 1 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HOLB_ECOLI DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1a5t, resolution 2.20Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=1a5t&oldid=4039917"