1a5t: Difference between revisions

← Older edit

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1a5t.gif|left|200px]]<br /><applet load="1a5t" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1a5t, resolution 2.2&Aring;" />
-'''CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III'''<br />
-==Overview==
+==CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III==
-The crystal structure of the delta' subunit of the clamp-loader complex of, E. coli DNA polymerase III has been determined. Three consecutive domains, in the structure are arranged in a C-shaped architecture. The N-terminal, domain contains a nonfunctional nucleotide binding site. The catalytic, component of the clamp-loader complex is the gamma subunit, which is, homologous to delta'. A sequence-structure alignment suggests that, nucleotides bind to gamma at an interdomain interface within the inner, surface of the "C." The alignment is extended to other clamp-loader, complexes and to the RuvB family of DNA helicases, and suggests that each, of these is assembled from C-shaped components that can open and close the, jaws of the "C" in response to ATP binding and hydrolysis.
+<StructureSection load='1a5t' size='340' side='right'caption='[[1a5t]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1a5t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A5T FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a5t OCA], [https://pdbe.org/1a5t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a5t RCSB], [https://www.ebi.ac.uk/pdbsum/1a5t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a5t ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/HOLB_ECOLI HOLB_ECOLI] DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/1a5t_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a5t ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-A5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Known structural/functional Site: <scene name='pdbsite=ZNB:Zn Binding Site'>ZNB</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A5T OCA].
+*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structure of the delta' subunit of the clamp-loader complex of E. coli DNA polymerase III., Guenther B, Onrust R, Sali A, O'Donnell M, Kuriyan J, Cell. 1997 Oct 31;91(3):335-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9363942 9363942]
+[[Category: Escherichia coli K-12]]
-[[Category: DNA-directed DNA polymerase]]
+[[Category: Large Structures]]
-[[Category: Escherichia coli]]
+[[Category: Guenther B]]
-[[Category: Single protein]]
+[[Category: Kuriyan J]]
-[[Category: Donnell, M.O.]]
+[[Category: O'Donnell M]]
-[[Category: Guenther, B.]]
+[[Category: Onrust R]]
-[[Category: Kuriyan, J.]]
+[[Category: Sali A]]
-[[Category: Onrust, R.]]
-[[Category: Sali, A.]]
-[[Category: ZN]]
-[[Category: dna replication]]
-[[Category: zinc finger]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 14:09:29 2007''