1a4f: Difference between revisions

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OCA

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-[[Image:1a4f.gif|left|200px]]<br />
-<applet load="1a4f" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1a4f, resolution 2.0&Aring;" />
-'''BAR-HEADED GOOSE HEMOGLOBIN (OXY FORM)'''<br />
-==Overview==
+==BAR-HEADED GOOSE HEMOGLOBIN (OXY FORM)==
-We have determined the crystal structure of bar-headed goose haemoglobin, in the oxy form to a resolution of 2.0 A. The R-factor of the model is, 19.8%. The structure is similar to human HbA, but contacts between the, subunits show slightly altered packing of the tetramer. Bar-headed goose, blood shows a greatly elevated oxygen affinity compared to closely related, species of geese. This is apparently due to a single proline to alanine, mutation at the alpha 1 beta 1 interface which destabilises the T state of, the protein. The beta chain N and C termini are well-localized, and, together with other neighbouring basic groups they form a strongly, positively charged groove at the entrance to the central cavity around the, molecular dyad. The well-ordered conformation and the three-dimensional, distribution of positive charges clearly indicate this area to be the, inositol pentaphosphate binding site of bird haemoglobins.
+<StructureSection load='1a4f' size='340' side='right'caption='[[1a4f]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1a4f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anser_indicus Anser indicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A4F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A4F FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a4f OCA], [https://pdbe.org/1a4f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a4f RCSB], [https://www.ebi.ac.uk/pdbsum/1a4f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a4f ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/HBA_ANSIN HBA_ANSIN] Involved in oxygen transport from the lung to the various peripheral tissues.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a4/1a4f_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a4f ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-A4F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Anser_indicus Anser indicus] with HEM and OXY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A4F OCA].
+*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-The crystal structure of a high oxygen affinity species of haemoglobin (bar-headed goose haemoglobin in the oxy form)., Zhang J, Hua Z, Tame JR, Lu G, Zhang R, Gu X, J Mol Biol. 1996 Jan 26;255(3):484-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8568892 8568892]
 [[Category: Anser indicus]]
-[[Category: Protein complex]]
+[[Category: Large Structures]]
-[[Category: Gu, X.]]
+[[Category: Gu X]]
-[[Category: Zhang, J.]]
+[[Category: Zhang J]]
-[[Category: HEM]]
-[[Category: OXY]]
-[[Category: erythrocyte]]
-[[Category: heme]]
-[[Category: oxygen transport]]
-[[Category: respiratory protein]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov  8 12:56:26 2007''