1a3k: Difference between revisions

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-[[Image:1a3k.gif|left|200px]]
-<!--
+==X-RAY CRYSTAL STRUCTURE OF THE HUMAN GALECTIN-3 CARBOHYDRATE RECOGNITION DOMAIN (CRD) AT 2.1 ANGSTROM RESOLUTION==
-The line below this paragraph, containing "STRUCTURE_1a3k", creates the "Structure Box" on the page.
+<StructureSection load='1a3k' size='340' side='right'caption='[[1a3k]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1a3k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A3K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A3K FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
--->
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-{{STRUCTURE_1a3k|  PDB=1a3k  |  SCENE=  }}
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a3k OCA], [https://pdbe.org/1a3k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a3k RCSB], [https://www.ebi.ac.uk/pdbsum/1a3k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a3k ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/LEG3_HUMAN LEG3_HUMAN] Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.<ref>PMID:15181153</ref> <ref>PMID:19594635</ref> <ref>PMID:19616076</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/1a3k_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a3k ConSurf].
+<div style="clear:both"></div>
-'''X-RAY CRYSTAL STRUCTURE OF THE HUMAN GALECTIN-3 CARBOHYDRATE RECOGNITION DOMAIN (CRD) AT 2.1 ANGSTROM RESOLUTION'''
+==See Also==
+*[[Galectin 3D structures|Galectin 3D structures]]
+== References ==
-==Overview==
+<references/>
-Galectins are a family of lectins which share similar carbohydrate recognition domains (CRDs) and affinity for small beta-galactosides, but which show significant differences in binding specificity for more complex glycoconjugates. We report here the x-ray crystal structure of the human galectin-3 CRD, in complex with lactose and N-acetyllactosamine, at 2.1-A resolution. This structure represents the first example of a CRD determined from a galectin which does not show the canonical 2-fold symmetric dimer organization. Comparison with the published structures of galectins-1 and -2 provides an explanation for the differences in carbohydrate-binding specificity shown by galectin-3, and for the fact that it fails to form dimers by analogous CRD-CRD interactions.
+__TOC__
+</StructureSection>
-==About this Structure==
-A3K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A3K OCA].
-==Reference==
-X-ray crystal structure of the human galectin-3 carbohydrate recognition domain at 2.1-A resolution., Seetharaman J, Kanigsberg A, Slaaby R, Leffler H, Barondes SH, Rini JM, J Biol Chem. 1998 May 22;273(21):13047-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9582341 9582341]
 [[Category: Homo sapiens]]
-[[Category: Single protein]]
+[[Category: Large Structures]]
-[[Category: Barondes, S H.]]
+[[Category: Barondes SH]]
-[[Category: Kanigsberg, A.]]
+[[Category: Kanigsberg A]]
-[[Category: Leffler, H.]]
+[[Category: Leffler H]]
-[[Category: Rini, J M.]]
+[[Category: Rini JM]]
-[[Category: Seetharaman, J.]]
+[[Category: Seetharaman J]]
-[[Category: Slaaby, R.]]
+[[Category: Slaaby R]]
-[[Category: Galaptin]]
-[[Category: Galectin]]
-[[Category: Ige-binding protein]]
-[[Category: Lectin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 09:45:36 2008''