197d: Difference between revisions

New page: left|200px<br /><applet load="197d" size="450" color="white" frame="true" align="right" spinBox="true" caption="197d, resolution 2.190Å" /> '''ORTHORHOMBIC CRYSTA...
 
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[[Image:197d.gif|left|200px]]<br /><applet load="197d" size="450" color="white" frame="true" align="right" spinBox="true"
caption="197d, resolution 2.190&Aring;" />
'''ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE'''<br />


==Overview==
==ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE==
The X-ray crystal structure of the double-helical A-DNA octanucleotide, d(GTACGTAC) has been solved by molecular replacement and refined to a, resolution of 0.219 nm. The final R-factor is equal to 16.1% for 1516, observed reflections with F &gt; 4 sigma(F). The sequence crystallizes as an, A-DNA-type double helix in the orthorhombic space group P2(1)2(1)2, with, one duplex molecule solvated by 66 water molecules in the asymmetric unit., Cell parameters are a = 3.860 nm, b = 5.082 nm, c = 2.174 nm. It is the, first time that such a crystal form has been observed. This orthorhombic, structure has been compared with the tetragonal structure of the same, oligonucleotide. It adopts a bent structure with an unusual packing, between symmetry-related molecules.
<StructureSection load='197d' size='340' side='right'caption='[[197d]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[197d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=197D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=197D FirstGlance]. <br>
197D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=197D OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=197d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=197d OCA], [https://pdbe.org/197d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=197d RCSB], [https://www.ebi.ac.uk/pdbsum/197d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=197d ProSAT]</span></td></tr>
==Reference==
</table>
Orthorhombic crystal structure of the A-DNA octamer d(GTACGTAC). Comparison with the tetragonal structure., Langlois d'Estaintot B, Dautant A, Courseille C, Precigoux G, Eur J Biochem. 1993 Apr 15;213(2):673-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8477741 8477741]
__TOC__
[[Category: Protein complex]]
</StructureSection>
[[Category: Courseille, C.]]
[[Category: Large Structures]]
[[Category: Dautant, A.]]
[[Category: Courseille C]]
[[Category: Estaintot, B.Langlois.D.]]
[[Category: Dautant A]]
[[Category: Precigoux, G.]]
[[Category: Langlois D'Estaintot B]]
[[Category: a-dna]]
[[Category: Precigoux G]]
[[Category: double helix]]
 
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